BIOL 1010 – Fall 2020
LAB # 3 – Enzymatic activity
Laboratory assignment
Name: Mariyah Ahmed
ID #: 100783593
TA’s name: Michael Jeffrey
Lab CRN #: 41955
For this laboratory assignment, perform the activities, watch the videos, fill in the
tables and answer the questions.
Write your answers on the lines provided. Anything written outside the space will
not be corrected.
Submit your assignment to Turnitin by the due date as a Word doc or docx file
or as a PDF file. No other format will be accepted.
Assignment due date are posted in The Laboratory Guidelines and in Turnitin.
1
, Part I: Investigate parameters that affect enzyme activities
Please use the virtual lab link provided in the protocol section
i. Effect of pH
Table 1. Effect of pH on enzymatic activity
Column 1 Column 2 Column 3
Optimum pH Acidic/basic or Relative activity
Enzymes
(+/- 0.1)*1 neutral*2 (in %) at pH 7*3
Acidic
1 4.5 1%
Basic
2 8.8 24%
Neutral
3 6.8 97%
Basic
4 9 4%
Acidic
5 2.5 0%
*1
Determined from the virtual lab
*2
Would the enzyme work best under acidic/ basic or neutral conditions?
(choose one of the 3 possibilities)
*3
From the curve obtained in the virtual lab, estimate the % enzyme activity at
pH 7
Answer the following questions:
What is the optimum pH of an enzyme (in general terms)?
This pH value has the most favorable condition for the enzyme to proceed at
maximum performance in the chemical reaction it is in.
Why is it important to perform enzymatic studies at or around the optimum pH
of the enzyme? Give three reasons:
Enzymes function most efficiently around a favored pH range, which is its
optimum pH value. It is crucial to perform enzymatic studies around the
optimum pH to avoid denaturation of the enzyme, disruption of the 3D compos-
ition of the protein and therefore, possible inactivation of enzyme activity.
2
LAB # 3 – Enzymatic activity
Laboratory assignment
Name: Mariyah Ahmed
ID #: 100783593
TA’s name: Michael Jeffrey
Lab CRN #: 41955
For this laboratory assignment, perform the activities, watch the videos, fill in the
tables and answer the questions.
Write your answers on the lines provided. Anything written outside the space will
not be corrected.
Submit your assignment to Turnitin by the due date as a Word doc or docx file
or as a PDF file. No other format will be accepted.
Assignment due date are posted in The Laboratory Guidelines and in Turnitin.
1
, Part I: Investigate parameters that affect enzyme activities
Please use the virtual lab link provided in the protocol section
i. Effect of pH
Table 1. Effect of pH on enzymatic activity
Column 1 Column 2 Column 3
Optimum pH Acidic/basic or Relative activity
Enzymes
(+/- 0.1)*1 neutral*2 (in %) at pH 7*3
Acidic
1 4.5 1%
Basic
2 8.8 24%
Neutral
3 6.8 97%
Basic
4 9 4%
Acidic
5 2.5 0%
*1
Determined from the virtual lab
*2
Would the enzyme work best under acidic/ basic or neutral conditions?
(choose one of the 3 possibilities)
*3
From the curve obtained in the virtual lab, estimate the % enzyme activity at
pH 7
Answer the following questions:
What is the optimum pH of an enzyme (in general terms)?
This pH value has the most favorable condition for the enzyme to proceed at
maximum performance in the chemical reaction it is in.
Why is it important to perform enzymatic studies at or around the optimum pH
of the enzyme? Give three reasons:
Enzymes function most efficiently around a favored pH range, which is its
optimum pH value. It is crucial to perform enzymatic studies around the
optimum pH to avoid denaturation of the enzyme, disruption of the 3D compos-
ition of the protein and therefore, possible inactivation of enzyme activity.
2
