Practicals
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Microscopy Practical
1) Rotate the objective lenses so that the low power, eg ×10, is in line with the stage.
2) Turn the coarse focus so that the stage is as close to the objective lens as possible. You should not look through the
microscope to do this.
3) Place the microscope slide – either one you have prepared, or a permanent slide – on the stage. Line it up so that the
specimen – if you can see it – is in the centre of the stage, where the light passes through.
4) Focus the slide towards you by turning the coarse focus adjustment.
5) Draw a low power image or record a digital image of what you see. Then, rotate the objectives so that the high power
objective, eg ×40, is in line with the stage.
6) Bring the slide back into focus using the fine focus adjustment. If you do not succeed, go back to low power and refocus, then
try again.
The drawing should be labelled with the magnification under it . This is how it should look:
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