BIOD 171 AM04 Intro to Growth Media

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BIOD 171 AM04 Intro to Growth Media




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AM04 Intro to Growth Media

Objective: To understand the types and uses of growth
media for the isolation and identification of unknown
bacterial samples.

Notes:

Non-selective media
 Non-selective media: important for the expansion of
unknown bacteria
o Anything will grow on media plates




m
er as
o Provides carbon source




co
 LB:



eH w
o Most common nutrient agar




o.
o Light yellow in color rs e
ou urc
o Place bacteria on the plate, invert it (turn upside
down) and store at 37
o

 Invert it so nothing settles on top of it
aC s


 Blood Agar:
vi y re



o Contains red blood cells
o Important as a nutrient source
ed d




o Tryptic soy agar yeast + red blood cells
ar stu




 TSAYE:
o Tryptic soy agar yeast extract
is




o If you remove the red blood cells
Th




o Nutrients: tryptic soy and yeast
Selective media
 Selective media: used to eliminate irrelevant
sh




bacteria from mixed cultures
 MacConkey:
o Slight cloudiness
o Red color


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