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MICROBIOLOGY MICROPATH PRE
EXAM 2021 (MODULE EXAMS AND
LABS ANSWERS.)
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PRE EXAM
1. A virus is classified as a microbe.
True. Although viruses are not living and as such are not considered microorganisms,
they can, however, be classified as microbes, a more general term that includes
microorganisms and viruses.
2. True or False: The smallest biological unit of life is the molecule.
False. The smallest biological unit of life is the cell.
3. What are the 4 main types of macromolecules found in cells?
Proteins, Lipids, Nucleic acids and Polysaccharides
1. Which of the following microorganisms are considered to be Eukarya? Select all that apply.
A. Animalia
B. Plantae
C. Fungi
D. Protista
A,B,C,D
2. True of False: All multicellular microorganisms classified as Animalia are heterotrophic.
True
3. Microorganisms classified as Plantae obtain most of their energy by converting _____ energy
into _______ energy.
Light (sunlight); chemical (sugars)
1. Define catabolism.
Catabolism is the process of breaking down larger molecules into useful energy sources.
2. Upon cellular injury, which metabolic process is involved during the growth and repair phases
of the cell?
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The anabolic process would be active as it (by definition) is involved in the building up of
small complexes into larger complexes.
1. In phosphorylation, the light reactions always occur where?
The process of converting light energy into chemical energy (photophosphorylation)
always occurs in the membrane.
2. True or False: The Calvin cycle must occur in the absence of light.
False. The term ‘dark reactions’ (also known as the Calvin Cycle) simply denotes the
second stage in photosynthesis—dark reactions do not actually require darkness in
order to occur.
1. Assuming a constant (non-adjustable) light source power, identify the part of the microscope
you would adjust to limit the amount of light entering the microscope.
Select all that apply.
A. Objective
B. Condenser
C. Iris diaphragm
D. Eye piece
C. The iris controls the amount of light that passes through the sample and into the
objective lens. Thus, it can be adjusted (opened or closed) to alter the amount of light.
2. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective
and a 10x eyepiece? Show your math.
60 x 10 = 600x magnification
1. Identify what type of electron microscope was used to capture the following image and
explain your choice.
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The above image is captured via a Transmission Electron Microscope (TEM). Even at
20nm resolution (inset image), subcellular substructures are still visible. The image lacks
the outside ‘shell’ only appearance of SEM.
1. True or False: LB agar is classified as a selective, non-differential media.
False. LB agar is the most basic type of agar and like LB media supports the growth of
virtually all microbes without restriction.
2. What is agar used for in microbiology?
Agar is used to create a solid, smooth surface on which microbes can grow.
1. True or False. When performing a dilution streak a new (or sterilized) loop is not required for
each phase as long as the bacterial culture is pure.
False. A new or sterilized loop is absolutely required for each phase. Failure to do so
would prevent the establishment of a dilution gradient, as the same bacterial
concentration would be spread across both phase regions, regardless of whether or not
the culture is pure.
2. The number of phases (3 vs. 4) and/or the number of times a loop passes through a previous
phase (once vs. multiple times) is acceptable provided what happens?
Either deviation is acceptable in practice provided the resulting gradient contains within
it the growth of individual colonies—if not, the experiment must be repeated.
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MICROBIOLOGY MICROPATH PRE
EXAM 2021 (MODULE EXAMS AND
LABS ANSWERS.)
Downloaded by: Kayyh |
Distribution of this document is illegal
, Stuvia.com - The Marketplace to Buy and Sell your Study Material
PRE EXAM
1. A virus is classified as a microbe.
True. Although viruses are not living and as such are not considered microorganisms,
they can, however, be classified as microbes, a more general term that includes
microorganisms and viruses.
2. True or False: The smallest biological unit of life is the molecule.
False. The smallest biological unit of life is the cell.
3. What are the 4 main types of macromolecules found in cells?
Proteins, Lipids, Nucleic acids and Polysaccharides
1. Which of the following microorganisms are considered to be Eukarya? Select all that apply.
A. Animalia
B. Plantae
C. Fungi
D. Protista
A,B,C,D
2. True of False: All multicellular microorganisms classified as Animalia are heterotrophic.
True
3. Microorganisms classified as Plantae obtain most of their energy by converting _____ energy
into _______ energy.
Light (sunlight); chemical (sugars)
1. Define catabolism.
Catabolism is the process of breaking down larger molecules into useful energy sources.
2. Upon cellular injury, which metabolic process is involved during the growth and repair phases
of the cell?
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The anabolic process would be active as it (by definition) is involved in the building up of
small complexes into larger complexes.
1. In phosphorylation, the light reactions always occur where?
The process of converting light energy into chemical energy (photophosphorylation)
always occurs in the membrane.
2. True or False: The Calvin cycle must occur in the absence of light.
False. The term ‘dark reactions’ (also known as the Calvin Cycle) simply denotes the
second stage in photosynthesis—dark reactions do not actually require darkness in
order to occur.
1. Assuming a constant (non-adjustable) light source power, identify the part of the microscope
you would adjust to limit the amount of light entering the microscope.
Select all that apply.
A. Objective
B. Condenser
C. Iris diaphragm
D. Eye piece
C. The iris controls the amount of light that passes through the sample and into the
objective lens. Thus, it can be adjusted (opened or closed) to alter the amount of light.
2. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective
and a 10x eyepiece? Show your math.
60 x 10 = 600x magnification
1. Identify what type of electron microscope was used to capture the following image and
explain your choice.
Downloaded by: Kayyh |
Distribution of this document is illegal
, Stuvia.com - The Marketplace to Buy and Sell your Study Material
The above image is captured via a Transmission Electron Microscope (TEM). Even at
20nm resolution (inset image), subcellular substructures are still visible. The image lacks
the outside ‘shell’ only appearance of SEM.
1. True or False: LB agar is classified as a selective, non-differential media.
False. LB agar is the most basic type of agar and like LB media supports the growth of
virtually all microbes without restriction.
2. What is agar used for in microbiology?
Agar is used to create a solid, smooth surface on which microbes can grow.
1. True or False. When performing a dilution streak a new (or sterilized) loop is not required for
each phase as long as the bacterial culture is pure.
False. A new or sterilized loop is absolutely required for each phase. Failure to do so
would prevent the establishment of a dilution gradient, as the same bacterial
concentration would be spread across both phase regions, regardless of whether or not
the culture is pure.
2. The number of phases (3 vs. 4) and/or the number of times a loop passes through a previous
phase (once vs. multiple times) is acceptable provided what happens?
Either deviation is acceptable in practice provided the resulting gradient contains within
it the growth of individual colonies—if not, the experiment must be repeated.
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