OPENSTAX MICROBIOLOGY TESTBANK- CHAPTER 13: CONTROL OF MICROBIAL GROWTH
OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
Chapter 13: Control of Microbial Growth
* = Correct answer
Multiple Choice
1. Which of the following is not an example of a fomite?
A. catheter
B. doorknob
C. mosquitoes*
D. towels
Difficulty: Easy
ASMStandard: 13, 14, 23
2. HEPA filters are required for which Biological Safety Level (BSL)?
A. BSL 1
B. BSL 2
C. BSL 3
D. BSL 4*
Difficulty: Easy
ASMStandard: 14, 37
3. From a clinical perspective, aseptic technique is carried out to do which of the following?
A. clean fomites
B. contaminate fomites
C. introduce contamination in living tissue
D. prevent contamination in living tissue*
Difficulty: Easy
ASMStandard: 36, 37
4. The autoclave uses which of the following mechanism(s) for sterilization?
A. pressure
B. temperature
C. pressure and temperature*
D. pressure, temperature, and pH
Difficulty: Easy
ASMStandard: 14, 36, 37
5. Autoclaves are designed to kill which of the following heat-resistant microbes?
A. endospores*
B. prions
C. vegetative bacterial and fungal cells
Page 1 of 17
,OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
D. viruses
Difficulty: Easy
ASMStandard: 14, 36, 37
6. The decimal reduction time (D-value) is how long it takes to kill which percentage of a
microbial population?
A. 10%
B. 50%
C. 90%*
D. 100%
Difficulty:Moderate
ASMStandard: 14
7. Which of the following actions would be most clearly considered bacteriostatic rather than
bactericidal?
A. autoclaving bacterial cultures
B. placing a bacterial culture in the −80 C freezer*
C. spraying 70% alcohol on a laboratory bench
D. treating a mattress with ethylene oxide
Difficulty:Moderate
ASMStandard: 14
8. Microbial death follows which type of trend?
A. linear
B. logarithmic*
C. power series
D. variable
Difficulty:Moderate
ASMStandard: 14
9. Which of the following are the standard conditions for an autoclave?
A. 100C, 1 psi, 60 minutes
B. 121C, 1 psi, 60 minutes
C. 121 C, 15 psi, 20 minutes*
D. 212C, 15 psi, 10 minutes
Difficulty:Moderate
ASMStandard: 14, 36, 37
10. Which of the following is nota milkborne pathogen?
A. Escherichia coli O157:H7
B. Listeria monocytogenes
Page 2 of 17
, OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
C. Salmonella enterica
D. Yersinia pestis*
Difficulty:Moderate
ASMStandard: 23
11. For appropriate food storage in refrigerators, the temperature should be set to no higher than
which of the following?
A. 5 C
B. 7 C*
C. 9 C
D. 11 C
Difficulty: Easy
ASM Standard: 14
12. How does pascalizationcontrol microbial growth?
A. by cross-linking proteins
B. by damaging DNA
C. by denaturing proteins*
D. by disrupting membrane integrity
Difficulty:Moderate
ASMStandard: 14
13. X-rays and gamma rays are examples of which type of radiation?
A. beta emission radiation
B. ionizing radiation*
C. nonionizing radiation
D. nonpenetrating radiation
Difficulty: Easy
ASMStandard: 14
14. Ultraviolet light is an example of which type of radiation?
A. beta emission radiation
B. ionizing radiation
C. nonionizing radiation*
D. penetrating radiation
Difficulty: Easy
ASMStandard: 14
15. To sterilize packaged intravenous tubing, which method would not be acceptable?
A. ethylene oxide
B. freezing
Page 3 of 17
OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
Chapter 13: Control of Microbial Growth
* = Correct answer
Multiple Choice
1. Which of the following is not an example of a fomite?
A. catheter
B. doorknob
C. mosquitoes*
D. towels
Difficulty: Easy
ASMStandard: 13, 14, 23
2. HEPA filters are required for which Biological Safety Level (BSL)?
A. BSL 1
B. BSL 2
C. BSL 3
D. BSL 4*
Difficulty: Easy
ASMStandard: 14, 37
3. From a clinical perspective, aseptic technique is carried out to do which of the following?
A. clean fomites
B. contaminate fomites
C. introduce contamination in living tissue
D. prevent contamination in living tissue*
Difficulty: Easy
ASMStandard: 36, 37
4. The autoclave uses which of the following mechanism(s) for sterilization?
A. pressure
B. temperature
C. pressure and temperature*
D. pressure, temperature, and pH
Difficulty: Easy
ASMStandard: 14, 36, 37
5. Autoclaves are designed to kill which of the following heat-resistant microbes?
A. endospores*
B. prions
C. vegetative bacterial and fungal cells
Page 1 of 17
,OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
D. viruses
Difficulty: Easy
ASMStandard: 14, 36, 37
6. The decimal reduction time (D-value) is how long it takes to kill which percentage of a
microbial population?
A. 10%
B. 50%
C. 90%*
D. 100%
Difficulty:Moderate
ASMStandard: 14
7. Which of the following actions would be most clearly considered bacteriostatic rather than
bactericidal?
A. autoclaving bacterial cultures
B. placing a bacterial culture in the −80 C freezer*
C. spraying 70% alcohol on a laboratory bench
D. treating a mattress with ethylene oxide
Difficulty:Moderate
ASMStandard: 14
8. Microbial death follows which type of trend?
A. linear
B. logarithmic*
C. power series
D. variable
Difficulty:Moderate
ASMStandard: 14
9. Which of the following are the standard conditions for an autoclave?
A. 100C, 1 psi, 60 minutes
B. 121C, 1 psi, 60 minutes
C. 121 C, 15 psi, 20 minutes*
D. 212C, 15 psi, 10 minutes
Difficulty:Moderate
ASMStandard: 14, 36, 37
10. Which of the following is nota milkborne pathogen?
A. Escherichia coli O157:H7
B. Listeria monocytogenes
Page 2 of 17
, OpenStaxMicrobiology Test Bank
Chapter 13: Control of Microbial Growth
C. Salmonella enterica
D. Yersinia pestis*
Difficulty:Moderate
ASMStandard: 23
11. For appropriate food storage in refrigerators, the temperature should be set to no higher than
which of the following?
A. 5 C
B. 7 C*
C. 9 C
D. 11 C
Difficulty: Easy
ASM Standard: 14
12. How does pascalizationcontrol microbial growth?
A. by cross-linking proteins
B. by damaging DNA
C. by denaturing proteins*
D. by disrupting membrane integrity
Difficulty:Moderate
ASMStandard: 14
13. X-rays and gamma rays are examples of which type of radiation?
A. beta emission radiation
B. ionizing radiation*
C. nonionizing radiation
D. nonpenetrating radiation
Difficulty: Easy
ASMStandard: 14
14. Ultraviolet light is an example of which type of radiation?
A. beta emission radiation
B. ionizing radiation
C. nonionizing radiation*
D. penetrating radiation
Difficulty: Easy
ASMStandard: 14
15. To sterilize packaged intravenous tubing, which method would not be acceptable?
A. ethylene oxide
B. freezing
Page 3 of 17