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BIOS 242 Week 3 iLab: Bacterial Growth and Controlling Bacterial Growth
Introduction
The case study I had was a 51-year old tourist arrived at the hospital in Buenos Aires
complaining of nausea and severe diarrhea. Doctors found a fever of 100.2 F, pulse rate
of 90, and blood pressure of 100/70. He was admitted to the hospital and placed on the
IV's to replace his fluids and balance his electrolytes. Blood, urine, and stool specimens
were obtained for analysis. This organism was recovered from the stool specimen. I
conducted a bacterial growth test along with an OF Glucose Test with and without Oil.
The purpose of these tests were to determine how the bacterium reacted to glucose with
and without oxygen. My findings reported a gram stain negative along with positive tests
for glucose oxidation and fermentation. The next step was an Indole production test. This
test determines whether the microbe produces indole from the amino acid tryptophan.
The results from this test were negative. The last test was Malonate utilization test.
Which the purpose of this is to see if the microbes can be use the compound malonate as
a sole source of carbon and energy of growth. Positive reaction.
Procedure: Conduct Bacterial Growth Test:
1. Click the New Unknown button. A window will open asking you to enter a label and
select a subgroup.
2. Type GrowthLab1 in the “Enter a Label” line.
3. From the Subgroup dropdown menu, select Growth.
, 2
4. Click Auto-inoculation allowed and then click OK.
5. Record details of your case study scenario.
6. The Gram Stain window will open. Record results of Gram stain.
7. Include the results in your Lab Report.
Conduct "OF Glucose Test":
1. Open the Biochemical Tests Reference Book. (You can find this resource in two way;
you can click on the T? button or select the Reference Books from the Help dropdown
menu.)
2. Open the OF Glucose Test. Review the steps of the test and determination of test
results before you begin your experiment.
3. From the Media dropdown menu, select OF Glucose Broth. The Media dropdown
menu is to the far right of the New Unknown button.
4. Enter OFGlucose1 in the Medium Label window to label your sample. Click OK. Two
tubes will appear. You will use the sample on the left to inoculate the sample on the right.
5. Turn on Bunsen burner by right clicking on the Bunsen burner and hitting on. You will
see the flame of the Bunsen burner.
6. Select Wire from the Tool dropdown menu. Flame the wire in the flame of the Bunsen
burner until it glows red.
7. Bring wire over to Inoculum sample. Right click on sample and select Inoculate.
8. Check the Traffic Signals in the upper right hand corner of the lab. Check to see that
you have successfully inoculated your sample and that no contamination was introduced.
9. Turn off Bunsen burner.
BIOS 242 Week 3 iLab: Bacterial Growth and Controlling Bacterial Growth
Introduction
The case study I had was a 51-year old tourist arrived at the hospital in Buenos Aires
complaining of nausea and severe diarrhea. Doctors found a fever of 100.2 F, pulse rate
of 90, and blood pressure of 100/70. He was admitted to the hospital and placed on the
IV's to replace his fluids and balance his electrolytes. Blood, urine, and stool specimens
were obtained for analysis. This organism was recovered from the stool specimen. I
conducted a bacterial growth test along with an OF Glucose Test with and without Oil.
The purpose of these tests were to determine how the bacterium reacted to glucose with
and without oxygen. My findings reported a gram stain negative along with positive tests
for glucose oxidation and fermentation. The next step was an Indole production test. This
test determines whether the microbe produces indole from the amino acid tryptophan.
The results from this test were negative. The last test was Malonate utilization test.
Which the purpose of this is to see if the microbes can be use the compound malonate as
a sole source of carbon and energy of growth. Positive reaction.
Procedure: Conduct Bacterial Growth Test:
1. Click the New Unknown button. A window will open asking you to enter a label and
select a subgroup.
2. Type GrowthLab1 in the “Enter a Label” line.
3. From the Subgroup dropdown menu, select Growth.
, 2
4. Click Auto-inoculation allowed and then click OK.
5. Record details of your case study scenario.
6. The Gram Stain window will open. Record results of Gram stain.
7. Include the results in your Lab Report.
Conduct "OF Glucose Test":
1. Open the Biochemical Tests Reference Book. (You can find this resource in two way;
you can click on the T? button or select the Reference Books from the Help dropdown
menu.)
2. Open the OF Glucose Test. Review the steps of the test and determination of test
results before you begin your experiment.
3. From the Media dropdown menu, select OF Glucose Broth. The Media dropdown
menu is to the far right of the New Unknown button.
4. Enter OFGlucose1 in the Medium Label window to label your sample. Click OK. Two
tubes will appear. You will use the sample on the left to inoculate the sample on the right.
5. Turn on Bunsen burner by right clicking on the Bunsen burner and hitting on. You will
see the flame of the Bunsen burner.
6. Select Wire from the Tool dropdown menu. Flame the wire in the flame of the Bunsen
burner until it glows red.
7. Bring wire over to Inoculum sample. Right click on sample and select Inoculate.
8. Check the Traffic Signals in the upper right hand corner of the lab. Check to see that
you have successfully inoculated your sample and that no contamination was introduced.
9. Turn off Bunsen burner.
