University of Texas, San Antonio BIOLOGY 1404-Lab 3 - Enzymes Post-Lab Assignment.

Results for Enzyme Specificity

Result Table 1. Enzyme Specificity

ENZ. A. B. C. D.
Enzyme Pork Rind H2O2 Urea p-nitrophenol
1 small piece 1ml 1ml phosphate
1ml
Observation Digestion Bubbling pH yellow color
ammonia smell
Enzyme 1 Buffer 1
No No pH10 No
3ml 3ml
Enzyme 2 Buffer 2
No Yes pH7 No
3ml 3ml

Enzyme 3 Buffer 3
No No pH7 No
3ml 3ml
Enzyme 4 Buffer 4
Yes No pH3 No
3ml 3ml
Enzyme 5 Buffer 5
No No pH7 Yes
3ml 3ml
6. No H2O
No No pH7 No
Enzyme 6ml


Question 1 What was the control in this experiment?
The Control in this experiment is #6 Enzyme.




Question 2 Was each enzyme specific for one substrate or was there some overlap? Why?
In this experiment, enzyme was specific for one substrate without overlap because they have a shap
substrates.




Question 3 Why does catalase bubble?
Catalase bubbles because it releases oxygen from H2O2?




Question 4 Why does urease reaction change pH?
Urease reaction changes pH converting Urease to Ammonia.

, Question 5 What would happen if you increased the enzyme concentration?
Increasing the enzyme concentration results in a faster reaction.




Question 6 What would happen if you increase the substrate concentration?
Increasing the substrate concentration results in a slower reaction.




Question 7 What would happen if another substance competed for the enzyme active site?
If another substance compete for the enzyme active site it will slow down the reaction influencing the