BIOS251 OL, Week 4 Lab
Name:
OL Lab 4: Microscopy
Learning Objectives:
• Understand different microscopy techniques and their limitations
• Identify various cell types and cellular structures
• Understand staining techniques
• Understand coeliac disease and intestinal inflammation
Introduction:
Histology is an important tool of biology which helps us understand how
tissues are structured and how they function. This is helpful when we are
investigating diseased or malformed tissues.
In this simulation you will examine a chicken intestine sample that is
infected with a retrovirus and observe how the virus infects the
lymphocytes (the cells that provide immune protection) and how it
inhibits inflammation. Inflammation is a sign of tissue damage. You will
use different microscopic techniques to examine chicken intestine slide.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to
see when using a light microscope—for example the microvilli. You can
zoom in and out to observe different cellular structures such as
mitochondria and the cell. Using the 5X magnification, you will identify the
villus, and then proceed with higher magnifications to identify smooth
muscle, extracellular tissue, epithelial cells, Goblet cells and the nuclei.
Learn about fluorescence staining techniques
For example, by staining the DNA with DAPI, you can easily identify a cell’s
nucleus. In this part of the lab, you will examine a chicken intestine sample
that is infected with a retrovirus and observe how the virus infects the
lymphocytes and how it inhibits inflammation. The retrovirus can be further
developed as medicine for coeliac disease.
, BIOS251 OL, Week 4 Lab
Name:
Part 1: Complete Labster Microscopy
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose
of this experiment.
The purpose of this experiment was to understand the components of
microscopic techniques. It was meant to observe the inflammation
effect the small intestine. You see how a virus affects cells.
Observations: List 3 observations you have made in this
simulation. 1.The infection of epithelial cells target the
lymphocytes cells to fight the viruses present in the epithelium.
2. The body does not fully activate its immune response to fight the virus
present if there is not an increase in lymphocytes cells in the body.
3. Celiac disease if the activation of immune response due to the
presence of gluten proteins in the intestinal tract. Resulting in the
body to inflame can cause damage to the body’s organs and tissues.
Answer all the questions below:
1. Distinguish the light microscope from the electron microscope.(1 point)
The light microscope uses a beam of light to produce the image of
a specimen and the electron uses a bean of electrons that
produces a higher resolution image.
2. Draw the three types of muscle tissue (3 points)
Smooth Skeletal muscle Cardiac
muscle
Name:
OL Lab 4: Microscopy
Learning Objectives:
• Understand different microscopy techniques and their limitations
• Identify various cell types and cellular structures
• Understand staining techniques
• Understand coeliac disease and intestinal inflammation
Introduction:
Histology is an important tool of biology which helps us understand how
tissues are structured and how they function. This is helpful when we are
investigating diseased or malformed tissues.
In this simulation you will examine a chicken intestine sample that is
infected with a retrovirus and observe how the virus infects the
lymphocytes (the cells that provide immune protection) and how it
inhibits inflammation. Inflammation is a sign of tissue damage. You will
use different microscopic techniques to examine chicken intestine slide.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to
see when using a light microscope—for example the microvilli. You can
zoom in and out to observe different cellular structures such as
mitochondria and the cell. Using the 5X magnification, you will identify the
villus, and then proceed with higher magnifications to identify smooth
muscle, extracellular tissue, epithelial cells, Goblet cells and the nuclei.
Learn about fluorescence staining techniques
For example, by staining the DNA with DAPI, you can easily identify a cell’s
nucleus. In this part of the lab, you will examine a chicken intestine sample
that is infected with a retrovirus and observe how the virus infects the
lymphocytes and how it inhibits inflammation. The retrovirus can be further
developed as medicine for coeliac disease.
, BIOS251 OL, Week 4 Lab
Name:
Part 1: Complete Labster Microscopy
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose
of this experiment.
The purpose of this experiment was to understand the components of
microscopic techniques. It was meant to observe the inflammation
effect the small intestine. You see how a virus affects cells.
Observations: List 3 observations you have made in this
simulation. 1.The infection of epithelial cells target the
lymphocytes cells to fight the viruses present in the epithelium.
2. The body does not fully activate its immune response to fight the virus
present if there is not an increase in lymphocytes cells in the body.
3. Celiac disease if the activation of immune response due to the
presence of gluten proteins in the intestinal tract. Resulting in the
body to inflame can cause damage to the body’s organs and tissues.
Answer all the questions below:
1. Distinguish the light microscope from the electron microscope.(1 point)
The light microscope uses a beam of light to produce the image of
a specimen and the electron uses a bean of electrons that
produces a higher resolution image.
2. Draw the three types of muscle tissue (3 points)
Smooth Skeletal muscle Cardiac
muscle
