Name:
OL Lab 3: Cell Membrane and Transport: Learn how transporters keep cells
healthy
Learning Objectives:
Describe the plasma membrane structure using the fluid mosaic model
Recognize the relative permeability of lipid bilayers to different classes of
molecule
Compare active and passive transport of molecules
Identify the 3 modes of active transport and the different classes of ion channel
and carrier molecules
Relate the expression of specific transport proteins to the cell’s role
Introduction:
A variety of substances move in and out of the cells through the plasma membrane.
Membrane transport is vital for cellular activities. The cells need to be healthy for the
body to work efficiently. From filtration in the kidneys to the contraction of muscles
during exercise, membrane transport contributes to many processes. In this simulation,
you will learn about the structure and function of the cell membrane, and discover why
membrane transporters are vital for healthy cells and the function of organ systems.
In the first part of your mission, you will be introduced to the concepts of selective
permeability and the fluid mosaic models of the plasma membrane. You will explore why
cells need specialized transporter proteins to transport cargo molecules across their
membranes. While some molecules can diffuse across the cell membrane, most
molecules require a transporter protein to enter or leave the cell.
In the later part of the simulation, you will set up a fluorescence microscopy experiment
to learn how the channels, carriers, and pumps that exist in the membrane ensures that
only the right molecules enter under the right conditions and help not only to keep the
cell healthy but also help organ systems to function.
Part 1: Complete Labster Cell Membrane and Transport
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, BIOS251 OL, Week 3 Lab
Name:
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of
this experiment.
The purpose of this experiment is to learn the active and passive transports of a cell
membrane.
Observations: List 2 observations you have made in this simulation.
The use of a microscope to visualize the molecules crossing through the cell
membrane.
The importance of keeping your lab sterile and avoiding cross-contamination of
your experiment.
Answer all the questions below:
1. In complete sentences and in your own words define the following cellular
transport: (2 points)
a. Diffusion:
Movement of molecules from greater concentration to lower concentration which
is driven by a gradient in concentration
b. Osmosis:
Movements of molecules from lower solute concentration to a higher solute
concentration to equalize the concentrations on each side of the membrane.
c. Facilitated diffusion:
The movement of specific materials across cell membrane through the protein
channels
d. Active transport:
The movement of molecules across a cell membrane from a region of lower
concentration to a region of higher concentration. This process requires cellular
energy to achieve this movement.
2. Using the figure below, identify the cellular structures and its function.(6 points)
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