Chapter 3 Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of
Microorganisms
Multiple Choice Questions
1. The Six I's of studying microorganisms include all of the following except A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
2. All of the following are examples of different types of microbiological media except A. broth.
B. enriched.
C. agar.
D. petri dish.
E. gelatin.
3. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
4. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
5. A pure culture contains only
A. one species of microorganism.
B. bacteria.
C. a variety of microbes from one source.
D. All of the choices are correct.
E. None of the choices are correct.
6. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
, 7. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following
incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be
growing in this culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
8. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture
medium. Following incubation, only the E. coli grows in the culture. What is the most likely
explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
9. Which method often results in colonies developing down throughout the agar and some
colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct
E. None of the choices are correct
10. A common medium used for growing fastidious bacteria is
A. blood agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.
11. A nutrient medium that has all of its chemical components identified and their precise
concentrations known and reproducible would be termed
A. complex.
B. reducing.
C. enriched.
D. enumeration.
E. synthetic.
3-2
Microorganisms
Multiple Choice Questions
1. The Six I's of studying microorganisms include all of the following except A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
2. All of the following are examples of different types of microbiological media except A. broth.
B. enriched.
C. agar.
D. petri dish.
E. gelatin.
3. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
4. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
5. A pure culture contains only
A. one species of microorganism.
B. bacteria.
C. a variety of microbes from one source.
D. All of the choices are correct.
E. None of the choices are correct.
6. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
, 7. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following
incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be
growing in this culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
8. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture
medium. Following incubation, only the E. coli grows in the culture. What is the most likely
explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.
9. Which method often results in colonies developing down throughout the agar and some
colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct
E. None of the choices are correct
10. A common medium used for growing fastidious bacteria is
A. blood agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.
11. A nutrient medium that has all of its chemical components identified and their precise
concentrations known and reproducible would be termed
A. complex.
B. reducing.
C. enriched.
D. enumeration.
E. synthetic.
3-2
