1. Staining is a commonly used microbiological technique that is
used for which of the following? Check all that apply.
Visualization of microorganisms, Differentiation of
microorganisms, Identification of some bacterial structures.
2. Staining allows for the visualization of bacterial cells because it
increases contrast during microscopic imaging. true
3. Staining techniques do NOT provide info on which of the
following structures? Ribosomes, Cell membrane.
4. Bacterial cells that are rod-shaped are called bacilli
5. Which of the images represents bacilli? B
6. Staining is a commonly used microbiological technique that is
used for which of the following? Visualization of microorganisms,
Differentiation of microorganisms.
7. To prepare a ____, a sample of bacteria is taken from a culture and
spread onto a clean microscope slide. smear
8. What is the purpose of the smear prep? To allow for visualization
of individual bacteria.
9. Which of the following could be determined through simple
staining of a microbial smear? Cellular arrangement, Cell
morphology.
10. Which of the following are characteristics of simple stains?
used to identify cellular arrangement of bacteria, used to visualize
morphology of bacteria.
, 11. Before bacteria from a solid culture are applied to a slide, a
drop of sterile water is typically added to the slide in order to?
Help the bacteria spread evenly, Reduce the tendency of the
bacteria to stick together.
12. Which of the following is true regarding proper heat fixation?
Heating too briefly may result in bacteria not adhering to the slide.
13. Which would be the proper amount of time required for
methylene blue dye to remain on the bacterial smear during a
simple stain? 30 seconds-2 minutes
14. Which of the following is/are the most appropriate way(s) to
correctly label the name of an organism on a glass slide? E. coli
15. How do you smear a bacterial culture onto the microscope
glass slide? Using the loop, you spread the bacteria onto the center
if the microscope slide
16. Which of the following describes the correct method for heat
fixation? The heating should be very brief. Passing the slide
though the flame for 3-5 seconds is enough
17. Which of the following would be appropriate for choosing a
sample to perform a smear preparation? Select all that apply. Use
bacteria from an isolated colony; Use only a small amount of
bacteria on the loop (when performing a smear preparation, use
bacteria from a single isolated colony)
used for which of the following? Check all that apply.
Visualization of microorganisms, Differentiation of
microorganisms, Identification of some bacterial structures.
2. Staining allows for the visualization of bacterial cells because it
increases contrast during microscopic imaging. true
3. Staining techniques do NOT provide info on which of the
following structures? Ribosomes, Cell membrane.
4. Bacterial cells that are rod-shaped are called bacilli
5. Which of the images represents bacilli? B
6. Staining is a commonly used microbiological technique that is
used for which of the following? Visualization of microorganisms,
Differentiation of microorganisms.
7. To prepare a ____, a sample of bacteria is taken from a culture and
spread onto a clean microscope slide. smear
8. What is the purpose of the smear prep? To allow for visualization
of individual bacteria.
9. Which of the following could be determined through simple
staining of a microbial smear? Cellular arrangement, Cell
morphology.
10. Which of the following are characteristics of simple stains?
used to identify cellular arrangement of bacteria, used to visualize
morphology of bacteria.
, 11. Before bacteria from a solid culture are applied to a slide, a
drop of sterile water is typically added to the slide in order to?
Help the bacteria spread evenly, Reduce the tendency of the
bacteria to stick together.
12. Which of the following is true regarding proper heat fixation?
Heating too briefly may result in bacteria not adhering to the slide.
13. Which would be the proper amount of time required for
methylene blue dye to remain on the bacterial smear during a
simple stain? 30 seconds-2 minutes
14. Which of the following is/are the most appropriate way(s) to
correctly label the name of an organism on a glass slide? E. coli
15. How do you smear a bacterial culture onto the microscope
glass slide? Using the loop, you spread the bacteria onto the center
if the microscope slide
16. Which of the following describes the correct method for heat
fixation? The heating should be very brief. Passing the slide
though the flame for 3-5 seconds is enough
17. Which of the following would be appropriate for choosing a
sample to perform a smear preparation? Select all that apply. Use
bacteria from an isolated colony; Use only a small amount of
bacteria on the loop (when performing a smear preparation, use
bacteria from a single isolated colony)
