1. Microorganisms may be found in which of the following
locations? Check all that apply on your hands; in the air in the
laboratory; on a disinfected bench top
2. The term “ubiquitous” refers to the fact that microorganisms are
naturally found in nearly every environment. true
3. What factors can greatly impact the growth of bacteria in an
environment or on a particular object? Check all that apply oxygen
availability; surface material and texture; pH; temperature;
moisture
4. Why are plates labeled on the bottom of the agar plate? Check all
that apply Plates are stored on their lids so this makes it easy to see
labels even when on the shelf of the incubator; the information
about the plate is still available even if the lid becomes separated
from the bottom; labeling the bottom allows labeling of different
sections of the plate. The lid might rotate and labels would not
match the sections.
5. Which image represents the correct position of an agar plate during
incubation? With the lid down
6. A standard temperature used for most incubations of bacterial
cultures in the clinical microbiology lab is _____ degrees Celsius
37
7. You accidentally drop a stack of agar plates as you take them to
the lab bench for observation. A few lids were separated from the
, plates. You may still be able to evaluate your results if the plates
were labeled on the bottom
8. The presence of opaque “spots” on the surface of an exposed agar
plate indicates that bacteria that were present in/on the exposed
environment are growing
9. Which of the following environments appears to exhibit the lowest
degree of microbial diversity? Alcohol washed fingers
10. What characteristics of bacteria/fungi are observable by
exposing, incubating, and examining the growth on agar plates?
Check all that apply Color of colonies; ability to be cultured on
general purpose media; colony morphology
11. Which of the following items are likely to show bacterial or
fungal growth if sampled onto a general-purpose medium? Check
all that apply doorknob, lab bench, fingers washed with water
12. The best time period to observe incubated cultures is
between___ hours 24-48
13. The use of ___ technique is important in obtaining
___cultures, those that contain only one type of microorganism
aseptic; pure
14. A pure culture contains one type of microorganism
locations? Check all that apply on your hands; in the air in the
laboratory; on a disinfected bench top
2. The term “ubiquitous” refers to the fact that microorganisms are
naturally found in nearly every environment. true
3. What factors can greatly impact the growth of bacteria in an
environment or on a particular object? Check all that apply oxygen
availability; surface material and texture; pH; temperature;
moisture
4. Why are plates labeled on the bottom of the agar plate? Check all
that apply Plates are stored on their lids so this makes it easy to see
labels even when on the shelf of the incubator; the information
about the plate is still available even if the lid becomes separated
from the bottom; labeling the bottom allows labeling of different
sections of the plate. The lid might rotate and labels would not
match the sections.
5. Which image represents the correct position of an agar plate during
incubation? With the lid down
6. A standard temperature used for most incubations of bacterial
cultures in the clinical microbiology lab is _____ degrees Celsius
37
7. You accidentally drop a stack of agar plates as you take them to
the lab bench for observation. A few lids were separated from the
, plates. You may still be able to evaluate your results if the plates
were labeled on the bottom
8. The presence of opaque “spots” on the surface of an exposed agar
plate indicates that bacteria that were present in/on the exposed
environment are growing
9. Which of the following environments appears to exhibit the lowest
degree of microbial diversity? Alcohol washed fingers
10. What characteristics of bacteria/fungi are observable by
exposing, incubating, and examining the growth on agar plates?
Check all that apply Color of colonies; ability to be cultured on
general purpose media; colony morphology
11. Which of the following items are likely to show bacterial or
fungal growth if sampled onto a general-purpose medium? Check
all that apply doorknob, lab bench, fingers washed with water
12. The best time period to observe incubated cultures is
between___ hours 24-48
13. The use of ___ technique is important in obtaining
___cultures, those that contain only one type of microorganism
aseptic; pure
14. A pure culture contains one type of microorganism
