BIOD 171: LABORATORY EXAMS Q & A
LAB VIII
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot to
do the wash step after Step D (above)?
, BIOD 171: LABORATORY EXAMS Q & A
All of the wells would have a dark color. The intensity of the readout is dependent
upon the amount of the labeled secondary antibody present in the well. Since the
unbound secondary antibody was not washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step
post-transfer?
Blocking prevents non-specific binding of the antibody—you only want the
antibody to bind to its specific target.
3. When developing a western blot what two factors influence the intensity of
the band?
Both the amount of protein loaded as well as the duration (time) of development is
directly proportional to band intensity.
4. Identify (specific name) given to the region of the western blot indicated by
the arrow.
The arrow is pointing to the dye front.
, BIOD 171: LABORATORY EXAMS Q & A
1. What is the common name for agglutination?
Clumping
, BIOD 171: LABORATORY EXAMS Q & A
2. In the wet lab portion of the video, what pathogen was the
agglutination assay specifically designed to test for? Choose all that
apply.
A. E coli
B. Salmonella
C. Staph
D. Strep
C. Staph
3. What was the purpose of the first quadrant of the agglutination
assay containing only the reagent?
The first quadrant served as the reagent only negative control—you want to
ensure the reagent by itself does not clump but rather requires the presence of
Staph.
4. Based on the image below, which row (A or B) is negative for agglutination?
Row A is negative for agglutination.
5. A patient with type O blood is sent to the lab for a blood-clotting
assessment. Since type O blood contains no antigens, which quadrant (#1-
LAB VIII
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot to
do the wash step after Step D (above)?
, BIOD 171: LABORATORY EXAMS Q & A
All of the wells would have a dark color. The intensity of the readout is dependent
upon the amount of the labeled secondary antibody present in the well. Since the
unbound secondary antibody was not washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step
post-transfer?
Blocking prevents non-specific binding of the antibody—you only want the
antibody to bind to its specific target.
3. When developing a western blot what two factors influence the intensity of
the band?
Both the amount of protein loaded as well as the duration (time) of development is
directly proportional to band intensity.
4. Identify (specific name) given to the region of the western blot indicated by
the arrow.
The arrow is pointing to the dye front.
, BIOD 171: LABORATORY EXAMS Q & A
1. What is the common name for agglutination?
Clumping
, BIOD 171: LABORATORY EXAMS Q & A
2. In the wet lab portion of the video, what pathogen was the
agglutination assay specifically designed to test for? Choose all that
apply.
A. E coli
B. Salmonella
C. Staph
D. Strep
C. Staph
3. What was the purpose of the first quadrant of the agglutination
assay containing only the reagent?
The first quadrant served as the reagent only negative control—you want to
ensure the reagent by itself does not clump but rather requires the presence of
Staph.
4. Based on the image below, which row (A or B) is negative for agglutination?
Row A is negative for agglutination.
5. A patient with type O blood is sent to the lab for a blood-clotting
assessment. Since type O blood contains no antigens, which quadrant (#1-
