DETAILED AND COMPLETE SOLUTIONS (GENOME
1.Complementary DNA &/or RNA molecules pair with each other by hydrogen bonding during
replication, transcription, or translation. Which pairs of molecules are complementary? Select all that
apply.
tRNA and mRNA
Template DNA and tRN
mRNA and template DNA
Coding and mRNA
(correct answers)tRNA and mRNA
mRNA and template DNA
2.The following sequence is one strand of the DNA that makes up the SNCA gene that codes for alpha-
synuclein, a protein found in the brain. 5' ATGCGGTGACGG 3' Which of the following is the
complementary second DNA strand? *
5' TACGCCACTGCC 3'
5' UACGCCACUGCC 3'
5' CCGTCACCGCAT 3'
5' CCGUCACCGCAT 3'
(correct answers)5' CCGTCACCGCAT 3'
3.Assume you start with one copy of target DNA. How many copies of DNA will be made after 4 rounds
of PCR (assume 100% reaction efficiency)?
2
, 8
16
32
(correct answers)16
4.What are the necessary ingredients for a PCR reaction? *
dNTPs; RNA polymerase; RNA primers; double-stranded DNA
dNTPs; thermostable DNA polymerase; RNA primers; single-stranded DNA
dNTPs; thermostable DNA polymerase; DNA primers; double-stranded DNA
dNTPs; RNA polymerase; DNA primers; single-stranded DNA (correct answers)
5.A frameshift mutation changed this sequence 5' AAC GCT TAG 3' to which of the following? *
5' AAC GCT TAA 3'
3' GAT TCG CA 5'
5' GAC GCT TAG 3'
3' AAC GCT TAG 5'
(correct answers)3' GAT TCG CA 5'
6.Which choice represents a silent mutation for this sequence: 5' GGU UGC ACU 3'?
5' GGU UGC ACU 3'
3' UCA CGU UAG 5'
5' GGU UGA ACU 3'
5' GGU UGC ACA 3'