A) Draw and label the set-up of Manual paraffin wax infiltration.
B) Answer the following questions:
1. Differentiate the 3 types of tissue Impregnation in terms of:
2. Impregnation Techniques Advantages Disadvantages
ADVANTAGES DISADVANTAGES
Paraffin 1. Thin sections may be cut easily 1. Overheating of paraffin results to a
Infiltration without tissue distortion. brittle/hard tissue specimen.
2. Rapid process (the process can be 2. Prolong impregnation makes the
completed within 24 hours). tissue shrink and hard, making the
3. The tissue blocks and unstained cutting of sections difficult.
mounted sections may be stored in 3. An inadequate impregnation will
paraffin for an indefinite period of time promote retention of the clearing