BIOS 390 – Molecular Biology
Week 2 Quiz Study Guide
Question Set 1
(TCO 3) DNA replication is said to be semiconservative because
Each daughter DNA molecule consists of one strand from the parent DNA
molecule and one new strand.
(TCO 3) DNA synthesis occurs in
The 5' to 3' direction.
(TCO 3) Which of the following statements is correct?
The growth and development or healing of wound in an organism require
new cells. The new cells in organism come from division of preexisting cells.
DNA is the material in body which has all structural and functional
information for body. Therefore each cell needs a full instruction in the form
of DNA to work properly. So before cell division the DNA need to be replicate
into two and transfer to new cell as a source of information.
The DNA replication occur before cell division in S-phase of cell cycle. The
DNA replication occur in nucleus of eukaryotic cell while prokaryotic cells do
not have nucleus and their cell division occur in cytoplasm.
Question Set 2
(TCO 3) During DNA replication, DNA ligase is most active on the lagging
strand.
This is because
The lagging strands contain more short DNA segments than the leading
strand, and these short segments are ligated together with DNA ligase
(TCO 3) Phosphodiester bonds are formed between adjacent Okazaki
fragments by
DNA ligase. DNA polymerase is the enzyme that carries in the daughter
nucleotides, and DNA helicase is the one that unwinds the double helix to
open the replication fork.
(TCO 3) Which of the following activities are unique for DNA polymerase I?
DNA polymerase I has 2 enzymatic function.
1. Polymerase activity
, 5' to 3' DNA dependent and RNA dependent polymerization.
2. Exonuclease activity
5' to 3' exonuclease during nick translation
3' to 5' exonuclease in proof reading.
Question Set 3
(TCO 3) Once bound to an origin of replication, the origin replication
complex (ORC) recruits
The first proteins to bind the DNA are said to “recruit” the other proteins.
Two copies of an enzyme called helicase are among the proteins recruited to
the origin. Each helicase unwinds and separates the DNA helix into single-
stranded DNA. As the DNA opens up, Y-shaped structures called replication
forks are formed.
(TCO 3) Positive supercoils ahead of the replication fork are resolved by
DNA gyrase.
Question Set 4
(TCO 3) Replication licensing ensures that
Control of DNA replication licensing in a cell cycle. To maintain genome
integrity in eukaryotes, DNA must be duplicated precisely once before cell
division occurs. A process called replication licensing ensures that
chromosomes are replicated only once per cell cycle.
(TCO 3) Pre-replication complex assembly
(TCO 3) Find the incorrect statement. (Describe the replication processes.)
Week 2 Quiz Study Guide
Question Set 1
(TCO 3) DNA replication is said to be semiconservative because
Each daughter DNA molecule consists of one strand from the parent DNA
molecule and one new strand.
(TCO 3) DNA synthesis occurs in
The 5' to 3' direction.
(TCO 3) Which of the following statements is correct?
The growth and development or healing of wound in an organism require
new cells. The new cells in organism come from division of preexisting cells.
DNA is the material in body which has all structural and functional
information for body. Therefore each cell needs a full instruction in the form
of DNA to work properly. So before cell division the DNA need to be replicate
into two and transfer to new cell as a source of information.
The DNA replication occur before cell division in S-phase of cell cycle. The
DNA replication occur in nucleus of eukaryotic cell while prokaryotic cells do
not have nucleus and their cell division occur in cytoplasm.
Question Set 2
(TCO 3) During DNA replication, DNA ligase is most active on the lagging
strand.
This is because
The lagging strands contain more short DNA segments than the leading
strand, and these short segments are ligated together with DNA ligase
(TCO 3) Phosphodiester bonds are formed between adjacent Okazaki
fragments by
DNA ligase. DNA polymerase is the enzyme that carries in the daughter
nucleotides, and DNA helicase is the one that unwinds the double helix to
open the replication fork.
(TCO 3) Which of the following activities are unique for DNA polymerase I?
DNA polymerase I has 2 enzymatic function.
1. Polymerase activity
, 5' to 3' DNA dependent and RNA dependent polymerization.
2. Exonuclease activity
5' to 3' exonuclease during nick translation
3' to 5' exonuclease in proof reading.
Question Set 3
(TCO 3) Once bound to an origin of replication, the origin replication
complex (ORC) recruits
The first proteins to bind the DNA are said to “recruit” the other proteins.
Two copies of an enzyme called helicase are among the proteins recruited to
the origin. Each helicase unwinds and separates the DNA helix into single-
stranded DNA. As the DNA opens up, Y-shaped structures called replication
forks are formed.
(TCO 3) Positive supercoils ahead of the replication fork are resolved by
DNA gyrase.
Question Set 4
(TCO 3) Replication licensing ensures that
Control of DNA replication licensing in a cell cycle. To maintain genome
integrity in eukaryotes, DNA must be duplicated precisely once before cell
division occurs. A process called replication licensing ensures that
chromosomes are replicated only once per cell cycle.
(TCO 3) Pre-replication complex assembly
(TCO 3) Find the incorrect statement. (Describe the replication processes.)
