BIOS 225 Lab Report Week 2- Transferring
Microbes While Using Aseptic Technique
Introduction:
Purpose: To learn an aseptic technique to reduce the minimum contamination, while
transferring bacteria from one place to another place by using inoculating loop or a needle, as
well as to isolate bacteria colony using the streak method.
Materials and Methods: Test tubes, bacteria (water), tops to test tubes, tube rack, LB agar
plates, media solution, Bunsen burner, and transfer loops. Safety equipment included goggles
and gloves.
Aseptic technique: a method designed to prevent contamination from microorganisms.
Results and Discussion: During this lab, the instructor explained how to use aseptic
techniques while transferring bacteria from one area to another. The instructor also
demonstrated how to transfer bacteria from tube to tube as well as from tube to agar plate using
aseptic technique. In order to practice aseptic technique and transfer bacteria, the following
steps must be followed.
Transferring from tube to tube steps using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Remove top from media tube, warm opening of media tube, then place bacteria
sample from the loop into media.
(e) Place top on media
(f) Use Bunsen burner to re-sterilize loop.
Transferring bacteria from tube to LB agar plate to conduct streak test using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Take top off LB agar plate and choose one side to do the first streak.
(e) Second streak, make sure to send loop through streak one.
(f) Third streak, make sure to send loop through streak two but not streak one.
(g) Fourth streak, make sure to send loop through streak three but do not touch first or
second streak. The fourth streak should be the most diluted streak.
(h) Place top on LB agar with streaks and put up to inoculate.
(i) Use Bunsen burner to re-sterilize loop.
Microbes While Using Aseptic Technique
Introduction:
Purpose: To learn an aseptic technique to reduce the minimum contamination, while
transferring bacteria from one place to another place by using inoculating loop or a needle, as
well as to isolate bacteria colony using the streak method.
Materials and Methods: Test tubes, bacteria (water), tops to test tubes, tube rack, LB agar
plates, media solution, Bunsen burner, and transfer loops. Safety equipment included goggles
and gloves.
Aseptic technique: a method designed to prevent contamination from microorganisms.
Results and Discussion: During this lab, the instructor explained how to use aseptic
techniques while transferring bacteria from one area to another. The instructor also
demonstrated how to transfer bacteria from tube to tube as well as from tube to agar plate using
aseptic technique. In order to practice aseptic technique and transfer bacteria, the following
steps must be followed.
Transferring from tube to tube steps using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Remove top from media tube, warm opening of media tube, then place bacteria
sample from the loop into media.
(e) Place top on media
(f) Use Bunsen burner to re-sterilize loop.
Transferring bacteria from tube to LB agar plate to conduct streak test using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Take top off LB agar plate and choose one side to do the first streak.
(e) Second streak, make sure to send loop through streak one.
(f) Third streak, make sure to send loop through streak two but not streak one.
(g) Fourth streak, make sure to send loop through streak three but do not touch first or
second streak. The fourth streak should be the most diluted streak.
(h) Place top on LB agar with streaks and put up to inoculate.
(i) Use Bunsen burner to re-sterilize loop.
