Lab 2 Introduction to Culturing & Aseptic
Technique “BIO250L”
Student Name:
Access Code (located on the lid of your lab kit):
“Experiment 1- Agar Plate Preparation and Bacterial Inoculation”
“Table 1: Experiment 1 Colony Growth”
“Plate
#” “Condition/Surface” “Growth (color, amount, shape, etc.)”
6 visible colonies. White. Circular in
“1” Toes
shape and one that is elongated.
2 visible colonies. Both are circle. One
“2” Child’s mouth
is orange and the other one is white.
TNTC. The colonies merged together.
Some look like web and some are
“3” Table
circular. Yellow and white in color, and
some are gray.
“4” control No visible colonies
Whiteish/yellowish, tiny growth, round
“5” Airborne Contamination
in shape
“Post-Lab Questions”
“1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a
surprise? Why or why not?”
Table #3 grew the most bacterial species. The kids have been eating and putting their toys on the table
the whole day so I wasn’t surprised that it had the most bacteria.
“2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If
not, what could be the source of the contamination?”
The control plate was free of bacteria because I was very careful when I was opening the plate to make
sure it was not getting contaminated.
“3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in
appearance to those found on the other plates? How does this plate demonstrate the need for
following aseptic technique when culturing bacteria?”
Yes it did but it was not too much growth. It turned a little yellow. There was less exposure to airborne
bacteria so it was not a lot of growth.
“Insert photo of your cultures after incubation with your name clearly visible in the background:”
Technique “BIO250L”
Student Name:
Access Code (located on the lid of your lab kit):
“Experiment 1- Agar Plate Preparation and Bacterial Inoculation”
“Table 1: Experiment 1 Colony Growth”
“Plate
#” “Condition/Surface” “Growth (color, amount, shape, etc.)”
6 visible colonies. White. Circular in
“1” Toes
shape and one that is elongated.
2 visible colonies. Both are circle. One
“2” Child’s mouth
is orange and the other one is white.
TNTC. The colonies merged together.
Some look like web and some are
“3” Table
circular. Yellow and white in color, and
some are gray.
“4” control No visible colonies
Whiteish/yellowish, tiny growth, round
“5” Airborne Contamination
in shape
“Post-Lab Questions”
“1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a
surprise? Why or why not?”
Table #3 grew the most bacterial species. The kids have been eating and putting their toys on the table
the whole day so I wasn’t surprised that it had the most bacteria.
“2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If
not, what could be the source of the contamination?”
The control plate was free of bacteria because I was very careful when I was opening the plate to make
sure it was not getting contaminated.
“3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in
appearance to those found on the other plates? How does this plate demonstrate the need for
following aseptic technique when culturing bacteria?”
Yes it did but it was not too much growth. It turned a little yellow. There was less exposure to airborne
bacteria so it was not a lot of growth.
“Insert photo of your cultures after incubation with your name clearly visible in the background:”
