“Lab 1 Introduction to Science BIO250L”
Student Name: Haley Leach
Access Code (located on the underside of the lid of your lab kit): AC-HMZEAY5
“Pre-Lab Questions”
1. What constitutes personal protective equipment? When should personal protective equipment
be worn? Why is personal protective equipment important?
PPE protects your skin and other sensitive areas like your nose, eyes, and mouth. It is a barrier
between you and protentailly harmful mircrorganisms, caustic chemicals, and biohazards. In this
lab I will be using safety goggles, gloves, and a lab coat. PPE should be worn at all times in the
lab.
2. Why are there increasing levels of biological containment procedures? What level of biological
containment will be necessary to complete the experiments in this manual?
The increasing levels of biological containment are warn and educate you on the degree of
danger that a biological pathogen may pose when you are handing it in the lab. Every level gives
a description, a sample organism, a pathogen type, and autoclave requirements. Only BSL-1
procedures will be used in this lab.
3. What document should you refer to if you have questions regarding the safety or disposal of a
chemical? Where can this document be located?
The material data safety sheet is found on the eScience Labs website.
4. List five safety procedures that should always be adhered to when working with microorganisms.
a. No snacking or chewing gum.
b. PPE must be worn at all time. (i.e. apron, gloves, mask)
c. Close toed shoes.
d. Long hair should be put up
e. Clean lab table/surface with 10% plase or isopropyl alcohol before and after experiments
, “Lab 1 Introduction to Science BIO250L”
Student Name: Haley Leach
Access Code (located on the underside of the lid of your lab kit): AC-HMZEAY5
“Pre-Lab Questions”
1. What constitutes personal protective equipment? When should personal protective equipment
be worn? Why is personal protective equipment important?
PPE protects your skin and other sensitive areas like your nose, eyes, and mouth. It is a barrier
between you and protentailly harmful mircrorganisms, caustic chemicals, and biohazards. In this
lab I will be using safety goggles, gloves, and a lab coat. PPE should be worn at all times in the
lab.
2. Why are there increasing levels of biological containment procedures? What level of biological
containment will be necessary to complete the experiments in this manual?
The increasing levels of biological containment are warn and educate you on the degree of
danger that a biological pathogen may pose when you are handing it in the lab. Every level gives
a description, a sample organism, a pathogen type, and autoclave requirements. Only BSL-1
procedures will be used in this lab.
3. What document should you refer to if you have questions regarding the safety or disposal of a
chemical? Where can this document be located?
The material data safety sheet is found on the eScience Labs website.
4. List five safety procedures that should always be adhered to when working with microorganisms.
a. No snacking or chewing gum.
b. PPE must be worn at all time. (i.e. apron, gloves, mask)
c. Close toed shoes.
d. Long hair should be put up
e. Clean lab table/surface with 10% plase or isopropyl alcohol before and after experiments
, “Lab 1 Introduction to Science BIO250L”
