Lab 11 Toxicology Experiment and Lab report
assignment
Project deliverables:
At the end of this project you will need to upload a copy of your lab assignment and report (see
guidelines below). You will upload your assignment into your Canvas course under the
“Toxicology experiment and lab report” module.
Overview
You are trying to determine the effect of salt toxicity on the sprouting of beans for the Office of
Pesticide Programs (OPP) at the Environmental Protection Agency (EPA). We are interested in
determining the toxicity of salt (between 0-1%) on beans. In other words, we want to know how
high a concentration of salt we can use without significantly impacting the germination rate of
beans.
Experimental Design
Formally, in order to apply the scientific method we need to:
State the problem
Develop hypotheses
Design and Perform Experiment
Analyze and interpret the results
Share the conclusions with others
First, read the scientific method background document in your Toxicology lab
module and answer questions 1-7 below.
State the problem: We must always have a specific thing that we are trying to measure and a
way to measure that hypothesis.
1. What specifically are we trying to find out with this experiment?
We are trying to know how high a concentration of salt we can use without significantly
impacting the germination rate of beans.
Develop hypotheses: We HAVE to have a very clear statement of what we think the
relationship between our dependent and independent variables will be. To do this we write
hypotheses. Hypotheses should be very clear and MUST ALWAYS BE TESTABLE!
2. What is the independent variable in this experiment?
The independent variable is the concentration of the salt.
This study source was downloaded by 100000839739811 from CourseHero.com on 04-18-2022 14:18:41 GMT -05:00
https://www.coursehero.com/file/67325220/Lab-11-toxicology-experiment-and-lab-report-exercise-online-version-1pdf/
, 3. What is the dependent variable in this experiment?
The dependent variable is the number of beans germinated.
4. Briefly describe the difference between an alternative hypothesis (HA) and a null
hypothesis (H0). What does each say about the relationship between our measured effect
(dependent variable) and our assumed cause (independent variable)?
An alternative hypothesis is a prediction that can be tested. This hypothesis states that their is a
relationship between the independent variable and dependent variable. The null hypothesis states
that their is no relationship between the independent variable and the dependent variable.
State the two hypotheses (null and alternative) for our salt/bean germination experiment
below:
5. H0:
There is no relationship between the the concentration of salt and number of beans that are
germinated.
6.
H A:
The higher concentration of salt added in the water will lower the number of beans that are
germinated.
7.
What endpoint are we going to measure to determine any effects? In other words, what will
be looking at in order to determine if our independent variable is affecting our dependent
variable?
If the number dependent variable lowers then we will know the independent variable has
changed the outcome we had originally predicted.
Note: These instructions below are just for your information, we are not
requiring you to perform the experiment but you need to know how it was
done in order to write a report about it (methodology).
Design and perform experiment (methods) : First you will design the experiment to
test for effects of salt concentration on bean germination. These beans usually have a 5-7 day
germination window, so the first week you will design and set up your experiment and next
This study source was downloaded by 100000839739811 from CourseHero.com on 04-18-2022 14:18:41 GMT -05:00
https://www.coursehero.com/file/67325220/Lab-11-toxicology-experiment-and-lab-report-exercise-online-version-1pdf/
assignment
Project deliverables:
At the end of this project you will need to upload a copy of your lab assignment and report (see
guidelines below). You will upload your assignment into your Canvas course under the
“Toxicology experiment and lab report” module.
Overview
You are trying to determine the effect of salt toxicity on the sprouting of beans for the Office of
Pesticide Programs (OPP) at the Environmental Protection Agency (EPA). We are interested in
determining the toxicity of salt (between 0-1%) on beans. In other words, we want to know how
high a concentration of salt we can use without significantly impacting the germination rate of
beans.
Experimental Design
Formally, in order to apply the scientific method we need to:
State the problem
Develop hypotheses
Design and Perform Experiment
Analyze and interpret the results
Share the conclusions with others
First, read the scientific method background document in your Toxicology lab
module and answer questions 1-7 below.
State the problem: We must always have a specific thing that we are trying to measure and a
way to measure that hypothesis.
1. What specifically are we trying to find out with this experiment?
We are trying to know how high a concentration of salt we can use without significantly
impacting the germination rate of beans.
Develop hypotheses: We HAVE to have a very clear statement of what we think the
relationship between our dependent and independent variables will be. To do this we write
hypotheses. Hypotheses should be very clear and MUST ALWAYS BE TESTABLE!
2. What is the independent variable in this experiment?
The independent variable is the concentration of the salt.
This study source was downloaded by 100000839739811 from CourseHero.com on 04-18-2022 14:18:41 GMT -05:00
https://www.coursehero.com/file/67325220/Lab-11-toxicology-experiment-and-lab-report-exercise-online-version-1pdf/
, 3. What is the dependent variable in this experiment?
The dependent variable is the number of beans germinated.
4. Briefly describe the difference between an alternative hypothesis (HA) and a null
hypothesis (H0). What does each say about the relationship between our measured effect
(dependent variable) and our assumed cause (independent variable)?
An alternative hypothesis is a prediction that can be tested. This hypothesis states that their is a
relationship between the independent variable and dependent variable. The null hypothesis states
that their is no relationship between the independent variable and the dependent variable.
State the two hypotheses (null and alternative) for our salt/bean germination experiment
below:
5. H0:
There is no relationship between the the concentration of salt and number of beans that are
germinated.
6.
H A:
The higher concentration of salt added in the water will lower the number of beans that are
germinated.
7.
What endpoint are we going to measure to determine any effects? In other words, what will
be looking at in order to determine if our independent variable is affecting our dependent
variable?
If the number dependent variable lowers then we will know the independent variable has
changed the outcome we had originally predicted.
Note: These instructions below are just for your information, we are not
requiring you to perform the experiment but you need to know how it was
done in order to write a report about it (methodology).
Design and perform experiment (methods) : First you will design the experiment to
test for effects of salt concentration on bean germination. These beans usually have a 5-7 day
germination window, so the first week you will design and set up your experiment and next
This study source was downloaded by 100000839739811 from CourseHero.com on 04-18-2022 14:18:41 GMT -05:00
https://www.coursehero.com/file/67325220/Lab-11-toxicology-experiment-and-lab-report-exercise-online-version-1pdf/
