WGU C785 BIOCHEMISTRY READINESS CHECK
Biochemistry Readiness Check
1B
The 2nd OA Readiness Check is designed to give you additional practice with
new assessment-style questions to help prepare for success on the OA. Thus,
please try to mimic exam-like conditions by taking the test in one <2 hr sitting
without any study aids apart from your whiteboard/scrap paper.
Note: Responses cannot be edited. You will be able to see the results and
feedback after submitting the test. A score of 70% is required for OA approval. If
you score below 70%, please speak with a Course Instructor via appointment to
discuss next steps prior to retaking this test.
Points: 67/67
1
What is the complementary sequence to 3’ CAG TAA CAA 5’?
(1/1 Point)
5' TTG TTA CTG 3
3’ GTC ATT GTT 5’
3’ GTT ATT GTC 5’
3' TTG TTA CTG 5
The correct answer is 3' TTG TTA CTG 5'. Remember complementary means “Matching
or Pairing” You have to remember to pay attention to your numbers as well as your
letters (A-T, G-C, 5'-3'). The correct answer is 3’ CAG TAA CAA 5’ (original sequence) 5’
GTC ATT GTT 3’ (complementary sequence) But we asked for it in the 3’ direction, so
mirror answer to give correct answer: 3’ TTG TTA CTG 5’
2
,Which amino acid sequence would result from this Coding DNA strand?
3’ TAC GTT AAC 5’?
(1/1 Point)
Gln Leu His
The correct answer is Gln Leu His. We are starting at the coding strand, and have to
remember the relationship between coding DNA and mRNA. These two strands are non-
complementary and parallel. So we copy the coding strand , change T ---> U, and then
write the mRNA sequence: 3’ TAC GTT AAC 5’ coding 3’ UAC GUU AAC 5’ mRNA Mirror
by changing orientation: 5’ CAA UUG CAU 3’ Read chart Gln Leu His (chart is in direction
of 5' ---> 3') If you chose Met Gln Leu, this answer is incorrect because this is the amino
acid sequence that results from the mRNA 5' AUG CAA UUG 3' which would have been
complementary to the given coding strand. Coding DNA is non-complementary and
parallel to mRNA.
Met Gln Leu
Ile Asp Val
Gln Ile Met
, 3
Which amino acid sequence would be made from this template DNA
strand? 5’ AGT TAT AAC 3’?
(1/1 Point)
Met Leu Thr
Val Ile Thr
The correct answer is Val Ile Thr because 5’ AGT TAT AAC 3’ template is complementary
and antiparallel so 3’ UCA AUA UUG 5’ but it is in the wrong orientation, so mirror 5’
GUU AUA ACU 3’ and read the chart Val Ile Thr
Ser Tyr Asn
Gln Tyr
4
Which ingredients/molecules are required to set up a polymerase chain
reaction? Select all that apply.
, (1/1 Point)
Amino Acids
RNA nucleotides
DNA polymerase
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
DNA nucleotides
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
Template DNA
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
DNA Primers
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
Ribosomes
RNA Primers
5
Assuming 100% reaction efficiency, how many DNA copies will be
produced after 5 PCR cycles, if we begin with 1 DNA template?
(1/1 Point)
Biochemistry Readiness Check
1B
The 2nd OA Readiness Check is designed to give you additional practice with
new assessment-style questions to help prepare for success on the OA. Thus,
please try to mimic exam-like conditions by taking the test in one <2 hr sitting
without any study aids apart from your whiteboard/scrap paper.
Note: Responses cannot be edited. You will be able to see the results and
feedback after submitting the test. A score of 70% is required for OA approval. If
you score below 70%, please speak with a Course Instructor via appointment to
discuss next steps prior to retaking this test.
Points: 67/67
1
What is the complementary sequence to 3’ CAG TAA CAA 5’?
(1/1 Point)
5' TTG TTA CTG 3
3’ GTC ATT GTT 5’
3’ GTT ATT GTC 5’
3' TTG TTA CTG 5
The correct answer is 3' TTG TTA CTG 5'. Remember complementary means “Matching
or Pairing” You have to remember to pay attention to your numbers as well as your
letters (A-T, G-C, 5'-3'). The correct answer is 3’ CAG TAA CAA 5’ (original sequence) 5’
GTC ATT GTT 3’ (complementary sequence) But we asked for it in the 3’ direction, so
mirror answer to give correct answer: 3’ TTG TTA CTG 5’
2
,Which amino acid sequence would result from this Coding DNA strand?
3’ TAC GTT AAC 5’?
(1/1 Point)
Gln Leu His
The correct answer is Gln Leu His. We are starting at the coding strand, and have to
remember the relationship between coding DNA and mRNA. These two strands are non-
complementary and parallel. So we copy the coding strand , change T ---> U, and then
write the mRNA sequence: 3’ TAC GTT AAC 5’ coding 3’ UAC GUU AAC 5’ mRNA Mirror
by changing orientation: 5’ CAA UUG CAU 3’ Read chart Gln Leu His (chart is in direction
of 5' ---> 3') If you chose Met Gln Leu, this answer is incorrect because this is the amino
acid sequence that results from the mRNA 5' AUG CAA UUG 3' which would have been
complementary to the given coding strand. Coding DNA is non-complementary and
parallel to mRNA.
Met Gln Leu
Ile Asp Val
Gln Ile Met
, 3
Which amino acid sequence would be made from this template DNA
strand? 5’ AGT TAT AAC 3’?
(1/1 Point)
Met Leu Thr
Val Ile Thr
The correct answer is Val Ile Thr because 5’ AGT TAT AAC 3’ template is complementary
and antiparallel so 3’ UCA AUA UUG 5’ but it is in the wrong orientation, so mirror 5’
GUU AUA ACU 3’ and read the chart Val Ile Thr
Ser Tyr Asn
Gln Tyr
4
Which ingredients/molecules are required to set up a polymerase chain
reaction? Select all that apply.
, (1/1 Point)
Amino Acids
RNA nucleotides
DNA polymerase
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
DNA nucleotides
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
Template DNA
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
DNA Primers
Correct! In PCR, a DNA sample is separated into single strands and incubated with DNA
polymerase, deoxynucleotides (dNTPs), and two short DNA primers whose sequences
flank the DNA segment of interest and thus define the region to be amplified. Recall
from the section on DNA replication that DNA polymerase needs a primer to begin DNA
synthesis. This requirement means the primers will direct the DNA polymerase to only
synthesize complementary strands of the target DNA.
Ribosomes
RNA Primers
5
Assuming 100% reaction efficiency, how many DNA copies will be
produced after 5 PCR cycles, if we begin with 1 DNA template?
(1/1 Point)
