Fungi MBK- Lab Report

MBK – Lab Report Name: April Tesch

Section:


Fungi
Part 1: Growing Fungal Cultures

Check the baggies at 24-hour intervals to monitor microbial growth.

Observe what has grown on your substrate. How many different types of
colonies can you see?

N/A

Part 2: Microscopic Observation – Wet Mount

Prepare a wet-mount of your 4 samples and observe with your
microscope.

Record your observations.

N/A

Part 3: Microscopic Observation – Simple Stain

Observe your stained specimen with your microscope. Record your
observations. Repeat for the remaining samples.

PCN with conidia at 40X – rods with spheres, more rods then spheres green
in color, rods more spaced the spheres, some spheres has opacity centers.
Yeast whole mount at 40X – spheres of purple dark clusters

Questions:

A. What is a hyphae? A mycelium?

Hyphae is a tube like structure with a rigid cell wall that protects the
cell membrane similar to a plant’s cell wall. Mycelium are thread-like
hyphae intertwine to form a tangled structure.

B. What is the difference between a septate hyphae and a non-septate
hyphae?

Septate hyphae have dividers between the cells, called septa. Non-
septate hyphae, also known as aseptate or coenocytic hyphae, form

, one long cell with many nuclei.