BIOCHEM C785 Final Exam
Which mRNA sequence results in a polypeptide that contains three amino acids?
5' - CUU GAU ACU UAG - 3'
5' - UAU GCC CUA CGA - 3'
5' - AUC CAG UAA AAG - 3'
5' - GUC UGA CAC CGG - 3' Correct Answer: 5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop
5' - UUC ACA GUA UAA - 3'
5' - UUU UAU GUU UGG - 3'
5' - UUC CAU UGA GUA - 3'
5' - AAU AUG ACA CUU - 3' Correct Answer: 5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
5' - CGG TAG AGC TTA - 3'
5' - ATT CGA GAT GGC - 3'
5' - TAA CGA GAT CCG - 3'
5' - CGG ATG TCG TTA - 3' Correct Answer: 5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four
complete PCR cycles?
24
16
8
4 Correct Answer: 16
What is the function of DNA polymerase in the process of PCR?
It separates the two DNA strands so that replication may occur.
It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence.
It synthesizes primers and uses the available dNTPs to replicate the template DNA sequence.
It links the products of PCR together to generate a complete strand. Correct Answer: It recognizes
the primers and uses the available dNTPs to replicate the template DNA sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA?
, A missense mutation leading to a longer protein
A nonsense mutation leading to a longer protein
A nonsense mutation leading to a shorter protein
A missense mutation leading to a shorter protein Correct Answer: A missense mutation leading to a
longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val).
What change in codons corresponds to this mutation?
GAA to GTA
GAA to GAT
GAC to GTC
GGU to GTT Correct Answer: GAA to GTA
What are possible blood types of the parents of a patient with blood type AB?
A and A
A and B
B and O
AB and O Correct Answer: A and B
Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated
with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to cocaine
increases their expression?
The DNA bases are chemically modified with methyl groups.
The nucleosomes become more widely spaced.
Transcription factors have less access to the promoter region.
RNA polymerase cannot bind better to the terminator region Correct Answer: The nucleosomes
become more widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese, while mice with
higher levels of expression are brown and of normal weight. Studies show that expression of the
agouti gene in mice can be controlled by epigenetics.
What scenario describes epigenetic regulation of the agouti gene?
Mother mice fed methyl-rich diets during pregnancy give birth to offspring with decreased agouti gene
expression compared to those fed a methyl-poor diet.
Mice with one allele in the agouti gene produce more agouti protein compared to mice with a different
allele.
A mother mouse exposed to radiation acquires a mutation in the agouti gene that makes a defective
agouti protein in her offspring.
A mouse that acquires a spontaneous mutation in a transcription factor is able to more easily activate
the agouti gene than mice without the mutation. Correct Answer: Mother mice fed methyl-rich diets
Which mRNA sequence results in a polypeptide that contains three amino acids?
5' - CUU GAU ACU UAG - 3'
5' - UAU GCC CUA CGA - 3'
5' - AUC CAG UAA AAG - 3'
5' - GUC UGA CAC CGG - 3' Correct Answer: 5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop
5' - UUC ACA GUA UAA - 3'
5' - UUU UAU GUU UGG - 3'
5' - UUC CAU UGA GUA - 3'
5' - AAU AUG ACA CUU - 3' Correct Answer: 5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
5' - CGG TAG AGC TTA - 3'
5' - ATT CGA GAT GGC - 3'
5' - TAA CGA GAT CCG - 3'
5' - CGG ATG TCG TTA - 3' Correct Answer: 5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four
complete PCR cycles?
24
16
8
4 Correct Answer: 16
What is the function of DNA polymerase in the process of PCR?
It separates the two DNA strands so that replication may occur.
It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence.
It synthesizes primers and uses the available dNTPs to replicate the template DNA sequence.
It links the products of PCR together to generate a complete strand. Correct Answer: It recognizes
the primers and uses the available dNTPs to replicate the template DNA sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA?
, A missense mutation leading to a longer protein
A nonsense mutation leading to a longer protein
A nonsense mutation leading to a shorter protein
A missense mutation leading to a shorter protein Correct Answer: A missense mutation leading to a
longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val).
What change in codons corresponds to this mutation?
GAA to GTA
GAA to GAT
GAC to GTC
GGU to GTT Correct Answer: GAA to GTA
What are possible blood types of the parents of a patient with blood type AB?
A and A
A and B
B and O
AB and O Correct Answer: A and B
Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated
with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to cocaine
increases their expression?
The DNA bases are chemically modified with methyl groups.
The nucleosomes become more widely spaced.
Transcription factors have less access to the promoter region.
RNA polymerase cannot bind better to the terminator region Correct Answer: The nucleosomes
become more widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese, while mice with
higher levels of expression are brown and of normal weight. Studies show that expression of the
agouti gene in mice can be controlled by epigenetics.
What scenario describes epigenetic regulation of the agouti gene?
Mother mice fed methyl-rich diets during pregnancy give birth to offspring with decreased agouti gene
expression compared to those fed a methyl-poor diet.
Mice with one allele in the agouti gene produce more agouti protein compared to mice with a different
allele.
A mother mouse exposed to radiation acquires a mutation in the agouti gene that makes a defective
agouti protein in her offspring.
A mouse that acquires a spontaneous mutation in a transcription factor is able to more easily activate
the agouti gene than mice without the mutation. Correct Answer: Mother mice fed methyl-rich diets
