Portage Microbiology Lab Exam 4
What type of media is best used to eliminate certain bacteria from within a mixed
culture? –
Selective media
If you were to remove the red bloods cells from at blood agar plate (BAP), what kind of
agar plate derivative would you have? –
TSAYE tryptic soy agar yeast extract
What was the name of the selective agar plate (shown below) that is similar to a blood
agar plate? –
MacConkey agar plate
What type of bacteria does MacConkey agar select for? –
SMAC selects gram negative bacteria
Sorbttol MacConkey (SMAC) agar is: -
Selective and differential media
What pathogen is best identified using a SMAC agar? –
E.coli standard K12 and E.coli 0157 that is a potent pathogen
What plating method is used to isolate a single bacteria colony? –
Four phase dilution streaking method
What plating method is used to grow multiple types of bacterial colonies? –
Quadrant Growth (non selective agar)
Based on the 4-phase dilution streaking experiment, in what phase were bacterial
isolates (colonies) observed? –
In phase or zone 3-4 we can see bacterial isolates or colonies
Identify the plating method as demonstrated in the lab –
4 quadrant growth method (multiple isolates)
Identify the organism growing on the BOTTOM half of the agar, and describe the
observed hemolytic properties. –
E.coli is growing on the bottom half of the agar plate. It doesn't have any lysis
properties (not beta hemolysis)
What color will E.coli exhibit when grown on an EMB agar plate? Explain your answer. –
What type of media is best used to eliminate certain bacteria from within a mixed
culture? –
Selective media
If you were to remove the red bloods cells from at blood agar plate (BAP), what kind of
agar plate derivative would you have? –
TSAYE tryptic soy agar yeast extract
What was the name of the selective agar plate (shown below) that is similar to a blood
agar plate? –
MacConkey agar plate
What type of bacteria does MacConkey agar select for? –
SMAC selects gram negative bacteria
Sorbttol MacConkey (SMAC) agar is: -
Selective and differential media
What pathogen is best identified using a SMAC agar? –
E.coli standard K12 and E.coli 0157 that is a potent pathogen
What plating method is used to isolate a single bacteria colony? –
Four phase dilution streaking method
What plating method is used to grow multiple types of bacterial colonies? –
Quadrant Growth (non selective agar)
Based on the 4-phase dilution streaking experiment, in what phase were bacterial
isolates (colonies) observed? –
In phase or zone 3-4 we can see bacterial isolates or colonies
Identify the plating method as demonstrated in the lab –
4 quadrant growth method (multiple isolates)
Identify the organism growing on the BOTTOM half of the agar, and describe the
observed hemolytic properties. –
E.coli is growing on the bottom half of the agar plate. It doesn't have any lysis
properties (not beta hemolysis)
What color will E.coli exhibit when grown on an EMB agar plate? Explain your answer. –
