Portage Microbiology Lab Exam 5 Questions And Answers 2022( With Complete Solution Rated A)

Portage Microbiology Lab Exam 5
The Kirby-Bauer method for examining antibiotic sensitivity is also known as what? –
Standardized diSC Susceptibility Test

True or False. The antibiotic discs are placed onto the LB agar plate before spreading
the bacterial culture on the plate –
false

When performing the Kirby-Bauer method the areas of clearing surrounding an
antibiotic disc after the overnight incubation are known as? –
Zone of inhibition

Why was an LB agar plate used to test the Staph culture as opposed to a
selective/differential agar that only grows Staph? –
Growing staph cultures on the lb agar plate will result in the yellow/gold coloured
colonies that will be easily identified.

What unit of measurement is used when determining the size of the zones of inhibition?
–
mm

True or False. In order to maintain proper spacing the antibiotic discs should be placed
a fingers width from the edge of the plate –
true

Given the following image, determine whether the bacterial sample is resistant or
susceptible to the following antibiotics –

A—24mm (because radius is given) SusceptibleB—9mm Susceptible, despite small
zone of inhibition.C—14mm Resistant