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Portage Learning BIO 171- Microbiology Lab Notebook LAB 1-9

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Portage Learning BIO 171- Microbiology Lab Notebook Lab Notebook Bookmarks (click to navigate): Lab 1 Notebook Lab 2 Notebook Lab 3 Notebook Lab 4 Notebook Lab 5 Notebook Lab 6 Notebook Lab 7 Notebook Lab 8 Notebook Lab 9 Notebook Lab 1 Notebook Back to Home Page Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation of samples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation of samples: microscopy- visualization and key characteristics of specimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure it is depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotates to aerateliquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria Outline of how to set up Notebook Objective: To establish an organized template for keeping experimental records, procedures, and results. Procedure: 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment- put them in red Notes: Additional (helpful) information placed here

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Portage Learning
BIO 171- Microbiology

Lab Notebook


Lab Notebook Bookmarks (click to navigate):

Lab 1 Notebook

Lab 2 Notebook

Lab 3 Notebook

Lab 4 Notebook

Lab 5 Notebook

Lab 6 Notebook

Lab 7 Notebook

Lab 8 Notebook

Lab 9 Notebook

, Lab 1 Notebook

Back to Home Page




Title: B, Lerner #PL01 Introduction to Medical Microbiology

Per graded lab, Your title should be BL01 Intro... You don't need to use PL anymore.

Objective: To understand course objectives, basic equipment used, as well as general lab safety
while working in a lab setting. Also, to understand how to create a lab notebook that is well
organized. All documentation should be recorded in a lab notebook while completing a lab.
Including the title, the objective (personal benefit to recall what was done during that lab), the
procedure (step by step instructions of what was done in that lab, along with any deviations
from the standard protocol), the notes (helpful information that does not fall into the procedure
section but that one would want to remember from the lab), and the results (the last section
which summarizes the findings during the lab and the main points of the lab).

Procedure:

1. Step by step protocol recorded here

a. Including:

i. What chemicals were used

ii. growth conditions

iii. temperature

iv. what type of plates were used?

2. Indicate any deviations that may occur during the experiment

a. Write any deviations in red (anything that is not standard protocol) that occurred

i. Deviations - something that absolutely stands out and is different than
the standard protocol

Notes: Additional (helpful) information, things that do not fall directly in the procedure

 Course Objectives –

, o Cultivation of samples (growth conditions)

 equipment used

 the cultivation of growth conditions of the samples in the lab settings

o Identification of samples (biochemical assays)

 tests available

 to look and determine what bacteria or what pathogens a patient may
have

o Evaluation of samples (microscopy)

 visualization and recognition of key variables (characteristics) of each
pathogen

 Basic Equipment

o Cleaning – working in a sterile environment to ensure everyone stays healthy

 Autoclave – device that uses heat, pressure, and steam equipment in a
lab to sterile equipment and also any samples that you might need to kill
after using them

 Temperature – above 125 degrees C

 Come in different sizes

 Does not just use dry air, uses hot steam

o steam is much more efficient than dry air to sterile
something

o Dry hot air takes a couple hours to achieve a sterile
condition

o Hot steam only will take a couple mins to achieve a sterile
environment

o Need to be very careful when opening autoclave when
finished using it because very hot steam will come out, so

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