Exam questions BBS2042 Cell
Signalling
Real exam questions with answers.
Note: these are real exam questions that I have seen in the 4 times I have tried passing this
exam (lol), they are all multiple choice or short answer open questions. Keep in mind that
the open-ended questions require a short answer of only 1 or 2 words.
,1. Nuclear receptors can function as transcription factors. How do such transcription
factors bind to the DNA?
A) With their DBD to a specific sequence
B) With their LBD to a specific sequence
C) With their LBD to a random sequence
D) With their DBD to a random sequence
2. Toxic compounds are removed from the body by phase I and phase II reactions.
What reactions below belong to phase I reactions? (multiple are correct)
- Oxidation
- Glutathion conjugation
- Acetylation
- Glucuronidation
- Sulfation
- Reduction
- Hydrolysis
3. An image of the RNAi process with 4 reaction steps is given below. What is de
correct sequence of the reactions?
A)
B)
C)
D)
Choose from: RISC loading process, Argonaute activity, guide strand pairing, dicer
activity
4. What of the answers below is/are functions of Nitric Oxide?
- Vasodilatator
- Killing bacteria
- Ligand for GPCRs
- Ligand for RTKs
- Neurotransmitter
, - Conversion of arginine to citrulline
5. Following binding to a nuclear receptor to DNA, several other proteins can be
recruited to the NR-DNA complex, like histone deacetylases. What is de effect of
HDAC’s when they are active in NR-DNA complexes?
A) The DNA becomes less accessible for the transcription machinery
B) The DNA becomes more accessible for the transcription machinery
C) The nuclear receptor becomes more activated
D) The nuclear receptor becomes more repressed
6. What is the correct sequence that explains tumor development adter exposure to a
chemical compound? Put the events below in the correct order
A)
B)
C)
D)
E)
F)
G)
Choose from: faulty DNAD repair, tumor development, induction of mutations,
exposure, bioactivation, activation of cell proliferation, DNA binding of compound
7. What is the result of mutating the HNH domain in Cas9 used in DNA modification kits
using the CRISPR system?
A) Hyperactivation of Cas9
B) Inactivation of Cas9
C) A single strand break
D) A double strand break
8. The Ah-receptor us involved in detoxicifcation of xenobiotic chemicals. Give the right
order of the dioxin detoxification steps mediated by Ah-R. Choose the correct order
from the options below.
A)
B)
C)
D)
E)
Choose from: translocation of AhR complex to the nucleus, AhR-ARNT heterodimer
formation, binding dioxin responding elements (DRE), AhR nuclear translocator
(ARNT) mediated HSP90 displacement, dioxine binds to the AhR.
9. As a biomedical researcher you are interested in cell signalling defects in cancer. You
work in a laboratory with access to SPR, MaMTH, FRET, phospoproteomics, CRISPR-
Cas9, and AP-MSMS. Match the correct technique to the given research techniques.
A) You want to study a real-time receptor-ligand interaction
B) You want to study the interaction between two cytosolic proteins
C) You want to study the interaction of two membrane-bound proteins
Signalling
Real exam questions with answers.
Note: these are real exam questions that I have seen in the 4 times I have tried passing this
exam (lol), they are all multiple choice or short answer open questions. Keep in mind that
the open-ended questions require a short answer of only 1 or 2 words.
,1. Nuclear receptors can function as transcription factors. How do such transcription
factors bind to the DNA?
A) With their DBD to a specific sequence
B) With their LBD to a specific sequence
C) With their LBD to a random sequence
D) With their DBD to a random sequence
2. Toxic compounds are removed from the body by phase I and phase II reactions.
What reactions below belong to phase I reactions? (multiple are correct)
- Oxidation
- Glutathion conjugation
- Acetylation
- Glucuronidation
- Sulfation
- Reduction
- Hydrolysis
3. An image of the RNAi process with 4 reaction steps is given below. What is de
correct sequence of the reactions?
A)
B)
C)
D)
Choose from: RISC loading process, Argonaute activity, guide strand pairing, dicer
activity
4. What of the answers below is/are functions of Nitric Oxide?
- Vasodilatator
- Killing bacteria
- Ligand for GPCRs
- Ligand for RTKs
- Neurotransmitter
, - Conversion of arginine to citrulline
5. Following binding to a nuclear receptor to DNA, several other proteins can be
recruited to the NR-DNA complex, like histone deacetylases. What is de effect of
HDAC’s when they are active in NR-DNA complexes?
A) The DNA becomes less accessible for the transcription machinery
B) The DNA becomes more accessible for the transcription machinery
C) The nuclear receptor becomes more activated
D) The nuclear receptor becomes more repressed
6. What is the correct sequence that explains tumor development adter exposure to a
chemical compound? Put the events below in the correct order
A)
B)
C)
D)
E)
F)
G)
Choose from: faulty DNAD repair, tumor development, induction of mutations,
exposure, bioactivation, activation of cell proliferation, DNA binding of compound
7. What is the result of mutating the HNH domain in Cas9 used in DNA modification kits
using the CRISPR system?
A) Hyperactivation of Cas9
B) Inactivation of Cas9
C) A single strand break
D) A double strand break
8. The Ah-receptor us involved in detoxicifcation of xenobiotic chemicals. Give the right
order of the dioxin detoxification steps mediated by Ah-R. Choose the correct order
from the options below.
A)
B)
C)
D)
E)
Choose from: translocation of AhR complex to the nucleus, AhR-ARNT heterodimer
formation, binding dioxin responding elements (DRE), AhR nuclear translocator
(ARNT) mediated HSP90 displacement, dioxine binds to the AhR.
9. As a biomedical researcher you are interested in cell signalling defects in cancer. You
work in a laboratory with access to SPR, MaMTH, FRET, phospoproteomics, CRISPR-
Cas9, and AP-MSMS. Match the correct technique to the given research techniques.
A) You want to study a real-time receptor-ligand interaction
B) You want to study the interaction between two cytosolic proteins
C) You want to study the interaction of two membrane-bound proteins
