WGU C785 OA Biochem Final Exam (New assessment style Q&A)

WGU C785 OA Biochem Final Exam (New assessment style Q&A) 1. What is the complementary sequence to 3’ ATG CGA ATA 5’? * 1/1 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ 2. Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? * 1/1 Tyr Val Tyr Ile Gln Ile Ile Asp Val Feedback The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ Leu His Lys 3. Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? * 0/1 Tyr Tyr Arg Ile Met Ala Ser Val Ile Ser His Gln Feedback The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T --- U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' --- 3') 4. Which of the following are the correct components for a PCR reaction? * 1/1 dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA ATP, Primer, mRNA polymerase; template mRNA Acetyl CoA, RNA primer, DNA Ligase, Template phosphate 5. If you had a mother who is homozygous for a recessive disease, and a father who is a heterozygous carrier, what percentage of children will have the disease? * 1/1 0% 25% 50% 75% 100% 6. Which of the following would represent a silent mutation if this is the original sequence: 5’ AUC GUA ACA 3’? * 1/1 Correct answer Ser Val Ile Feedback The correct answer is C because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile Feedback The correct answer is b Feedback The correct answer is c 50% Homozygous recessive: aa Heterozygous: Aa 5’ AUC GGA ACA 3’ 5’ AUA GUA ACA 3’ 5’ AUC GUA UAA 3’ 5’ AUC CUC ACA 3’ 7. If the original coding sequence is 5’ CGA TAC TTC AGA 3’ and it is mutated to 5' CGA TAT TTC AGA 3', what type of mutation would have taken place? * 1/1