HC6 DNA-analyse
RFLP
- Restriction Fragment Length Polymorphism
- Verschil in lengte van stukjes DNA die overblijven na een behandeling met enzymen
- Locatie waar enzym DNA breekt is overerfbaar.
- Ouderwetse techniek
PCR
Amplification
- PCR= polymerase chain reaction
- Process that copies a particular region of DNA using two ‘primers’ (short pieces of
DNA).
- Each strand of DNA is used as a template to create a replica that permits a doubling
of the number of target molecules with each cycle of heating and cooling.
Thermal Cycling
- The polymerase chain reaction (PCR) copies sections of DNA through heating and
cooling the sample.
- Each DNA strand is copied with each temperature cycle.
- A thermal cycler heats and cools DNA samples (usually 28 cycles).
Benodigdheden
- Polymerase
- Het vermeerderen van het DNA
- Hittebestendig vanwege de hoge temperatuur in PCR cyclus.
- Tag veelgebruikt
- Primers
- Beginpunten
- 1 voor 3’ en 1 voor 5’
- Uniek genoeg om maar 1 locus te amplificeren.
Reaction Setup
- DNA sample is added (about 1 ng based on DNA quantitation performed)
- PCR primers and other reaction chemicals from an STR typing kit are added.
RFLP
- Restriction Fragment Length Polymorphism
- Verschil in lengte van stukjes DNA die overblijven na een behandeling met enzymen
- Locatie waar enzym DNA breekt is overerfbaar.
- Ouderwetse techniek
PCR
Amplification
- PCR= polymerase chain reaction
- Process that copies a particular region of DNA using two ‘primers’ (short pieces of
DNA).
- Each strand of DNA is used as a template to create a replica that permits a doubling
of the number of target molecules with each cycle of heating and cooling.
Thermal Cycling
- The polymerase chain reaction (PCR) copies sections of DNA through heating and
cooling the sample.
- Each DNA strand is copied with each temperature cycle.
- A thermal cycler heats and cools DNA samples (usually 28 cycles).
Benodigdheden
- Polymerase
- Het vermeerderen van het DNA
- Hittebestendig vanwege de hoge temperatuur in PCR cyclus.
- Tag veelgebruikt
- Primers
- Beginpunten
- 1 voor 3’ en 1 voor 5’
- Uniek genoeg om maar 1 locus te amplificeren.
Reaction Setup
- DNA sample is added (about 1 ng based on DNA quantitation performed)
- PCR primers and other reaction chemicals from an STR typing kit are added.
