DAT lab study questions
what is the purpose of performing the DAT procedure? - Answer used to determine if
red cells have been coated in vivo with immunoglobulin, complement or both.
what does a positive DATE indicate? - Answer indicates that red cells are coated with
protein in-vivo
what 3 reagents may be used in the DAT procedure? - Answer polyspecific IgG, C3d
monospecific IgG or C3d
what is the sample of choice for the DAT? Why? - Answer whole blood collected in an
EDTA tube because it prevents in-vitro fixation of complement.
the DAT is used to assist in hemolysis associated with what 4 clinical conditions: -
Answer HDFN
autoimmune hemolytic anemia
red cell sensitization
transfusion rxns
what 3 items in the pt history are crucial in determining the clinical significance of a
positive DAT? - Answer pts diagnosis
transfusion and pregnancy hx
recent/current medications
state the principle of the DAT procedure - Answer the detection of abs that attached to
red cells in-vivo
what ab class is involved in HDFN? - Answer IgG
what is the source of the specimen used for the DAT on newborns? - Answer cord blood
sample
what is Wharton's Jelly? - Answer A gelatinous substance that provides insulation and
protection within the umbilical cord
what problems will contamination of the cord blood sample with Wharton's Jelly cause?
- Answer nonspecific agglutination of red cells, can cause a false positive result
list 3 types of HDFN from mildest to severe and state the ab specificity involved in each
- Answer 1. ABO: most common, mild, group A/B
2. Anti-D: most severe
what is the purpose of performing the DAT procedure? - Answer used to determine if
red cells have been coated in vivo with immunoglobulin, complement or both.
what does a positive DATE indicate? - Answer indicates that red cells are coated with
protein in-vivo
what 3 reagents may be used in the DAT procedure? - Answer polyspecific IgG, C3d
monospecific IgG or C3d
what is the sample of choice for the DAT? Why? - Answer whole blood collected in an
EDTA tube because it prevents in-vitro fixation of complement.
the DAT is used to assist in hemolysis associated with what 4 clinical conditions: -
Answer HDFN
autoimmune hemolytic anemia
red cell sensitization
transfusion rxns
what 3 items in the pt history are crucial in determining the clinical significance of a
positive DAT? - Answer pts diagnosis
transfusion and pregnancy hx
recent/current medications
state the principle of the DAT procedure - Answer the detection of abs that attached to
red cells in-vivo
what ab class is involved in HDFN? - Answer IgG
what is the source of the specimen used for the DAT on newborns? - Answer cord blood
sample
what is Wharton's Jelly? - Answer A gelatinous substance that provides insulation and
protection within the umbilical cord
what problems will contamination of the cord blood sample with Wharton's Jelly cause?
- Answer nonspecific agglutination of red cells, can cause a false positive result
list 3 types of HDFN from mildest to severe and state the ab specificity involved in each
- Answer 1. ABO: most common, mild, group A/B
2. Anti-D: most severe
