Biotechnology Exam 3
1) You just cloned a new gene from mice. Which of the following techniques would be
the best choice to use if you wanted to determine the number of copies of this gene in
the mouse genome? - Answer Southern Blot analysis
1) Which of the following techniques involves hybridizing a cDNA sample to a chip
containing thousands of single-stranded DNA sequences, allowing one to study the
expression of thousands of genes simultaneously? - Answer DNA microarray
1) When making a complementary DNA (cDNA) library, which enzyme is used to copy
mRNA into DNA? - Answer Reverse transcriptase
In a recombinant DNA experiment, which enzyme is used to join together DNA
fragments by forming phosphodiester bonds between nucleotides? Recall that this
same enzyme joins together Okazaki fragments on the lagging strand during DNA
replication. - Answer DNA ligase
1) During library screening, PCR, Southern blotting, and other techniques, binding two
pieces of DNA to each other by hydrogen bonding is called .
A) autoradiography - Answer hybridization
is a DNA-binding dye that fluoresces when DNA in an agarose gel is illuminated with
ultraviolet light - Answer Ethidium bromide
Which of the following vectors would be the best choice for gene transfer in plant cells?
- Answer Plasmid
A is a single-stranded DNA molecule attached to a radioactive or fluorescent compound
that is complementary to a specific sequence of DNA. Such pieces of DNA are used to
identify and study cloned genes in hybridization experiments. - Answer Probe
Transformation in a cloning experiment is - Answer inserting DNA into bacteria cells
Which of the following is an INCORRECT statement about restriction enzymes?
A) Most restriction enzymes are isolated from bacteria.
B) Restriction enzymes usually recognize palindromic sequences.
C) Restriction enzymes create phosphodiester bonds between pieces of DNA in a
cloning experiment.
D) Restriction enzymes can cut to create overlapping single-stranded ends of DNA.
Restriction enzymes can cut to create blunt-ended pieces ofDNA. - Answer A)
Restriction enzymes create phosphodiester bonds between pieces of DNA in a cloning
experiment.
, 1) Based on what you know about which organisms naturally produce restriction
enzymes and how restriction enzymes are named, which of the following enzymes is
isolated from the bacterium Bacillus amyloliquefaciens? - Answer BamH1
1) Imagine you wanted to use a human genomic DNA library to clone the human gene
for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what
order would you perform the following steps to accomplish this goal?
1. Use autoradiography to identify colonies containing DNA that hybridized to the probe
2. Grow transformed cells on media with antibiotics and X-gal for blue-white screening
3. Ligate genomic DNA and vector DNA
4. Cut genomic DNA and vector DNA with restriction enzymes
5. Hybridize library DNA with labeled probe for the rat insulin gene
6. Transform bacteria with recombinant plasmid - Answer 4, 3, 6, 2, 5, 1
Approximately how large is the human genome? - Answer 3.1 billion bp
Approximately how many genes are present in the human genome? - Answer 20,000
genes
Which of the following techniques is the best choice for amplifying DNA? - Answer PCR
1) Which of the following techniques is most commonly used to separate and analyze
DNA by size? - Answer Agarose gel electrophoresis
1) A consists of cloned DNA fragments for all expressed genes in a particular tissue -
Answer cDNA library
1) Which of the following techniques is most efficient for transforming bacterial cells? -
Answer Electroporation
1) Which of the following statements about the human genome is INCORRECT?
A) There are approximately 3.1 billion bp in the human genome.
B) Human share a majority of genes with other species.
C) Less than 2 percent of the genome codes for proteins.
D) The human genome contains approximately 100,000 genes.
The functions of most human genes are still unknown - Answer D
Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at -
Answer the 2' and 3' carbons of the pentose sugar
A recombinant DNA molecule is produced artificially and contains sequences - Answer
from unrelated organisms
A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction
enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA
1) You just cloned a new gene from mice. Which of the following techniques would be
the best choice to use if you wanted to determine the number of copies of this gene in
the mouse genome? - Answer Southern Blot analysis
1) Which of the following techniques involves hybridizing a cDNA sample to a chip
containing thousands of single-stranded DNA sequences, allowing one to study the
expression of thousands of genes simultaneously? - Answer DNA microarray
1) When making a complementary DNA (cDNA) library, which enzyme is used to copy
mRNA into DNA? - Answer Reverse transcriptase
In a recombinant DNA experiment, which enzyme is used to join together DNA
fragments by forming phosphodiester bonds between nucleotides? Recall that this
same enzyme joins together Okazaki fragments on the lagging strand during DNA
replication. - Answer DNA ligase
1) During library screening, PCR, Southern blotting, and other techniques, binding two
pieces of DNA to each other by hydrogen bonding is called .
A) autoradiography - Answer hybridization
is a DNA-binding dye that fluoresces when DNA in an agarose gel is illuminated with
ultraviolet light - Answer Ethidium bromide
Which of the following vectors would be the best choice for gene transfer in plant cells?
- Answer Plasmid
A is a single-stranded DNA molecule attached to a radioactive or fluorescent compound
that is complementary to a specific sequence of DNA. Such pieces of DNA are used to
identify and study cloned genes in hybridization experiments. - Answer Probe
Transformation in a cloning experiment is - Answer inserting DNA into bacteria cells
Which of the following is an INCORRECT statement about restriction enzymes?
A) Most restriction enzymes are isolated from bacteria.
B) Restriction enzymes usually recognize palindromic sequences.
C) Restriction enzymes create phosphodiester bonds between pieces of DNA in a
cloning experiment.
D) Restriction enzymes can cut to create overlapping single-stranded ends of DNA.
Restriction enzymes can cut to create blunt-ended pieces ofDNA. - Answer A)
Restriction enzymes create phosphodiester bonds between pieces of DNA in a cloning
experiment.
, 1) Based on what you know about which organisms naturally produce restriction
enzymes and how restriction enzymes are named, which of the following enzymes is
isolated from the bacterium Bacillus amyloliquefaciens? - Answer BamH1
1) Imagine you wanted to use a human genomic DNA library to clone the human gene
for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what
order would you perform the following steps to accomplish this goal?
1. Use autoradiography to identify colonies containing DNA that hybridized to the probe
2. Grow transformed cells on media with antibiotics and X-gal for blue-white screening
3. Ligate genomic DNA and vector DNA
4. Cut genomic DNA and vector DNA with restriction enzymes
5. Hybridize library DNA with labeled probe for the rat insulin gene
6. Transform bacteria with recombinant plasmid - Answer 4, 3, 6, 2, 5, 1
Approximately how large is the human genome? - Answer 3.1 billion bp
Approximately how many genes are present in the human genome? - Answer 20,000
genes
Which of the following techniques is the best choice for amplifying DNA? - Answer PCR
1) Which of the following techniques is most commonly used to separate and analyze
DNA by size? - Answer Agarose gel electrophoresis
1) A consists of cloned DNA fragments for all expressed genes in a particular tissue -
Answer cDNA library
1) Which of the following techniques is most efficient for transforming bacterial cells? -
Answer Electroporation
1) Which of the following statements about the human genome is INCORRECT?
A) There are approximately 3.1 billion bp in the human genome.
B) Human share a majority of genes with other species.
C) Less than 2 percent of the genome codes for proteins.
D) The human genome contains approximately 100,000 genes.
The functions of most human genes are still unknown - Answer D
Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at -
Answer the 2' and 3' carbons of the pentose sugar
A recombinant DNA molecule is produced artificially and contains sequences - Answer
from unrelated organisms
A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction
enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA
