BIOD 171 MICROLABS 1-9
BIOD 171 MICROLABS 1-9
TK01 Keeping a Lab Notebook
Microbiology Lab1
Course Objectives:
Cultivation of samples (growth conditions) - equipment used
Identification of samples (biochemical assays) - tests available
Evaluation of samples (microscopy) - visualizations and recognitions key concepts
Basic Equipment:
1. Cleaning: autoclave 125 ° C, steamed, heat and pressure sterilization, chamber with thick
door, not dry air but steamed - more efficient, make sure depressurized before opening
Time restrictions: no autoclave then use steam (minutes) vs. hot air (hours)
2. Growing: fixed incubator = 37 ° C; shaker incubator = 37 ° C, for growth mediator, rotates and
shakes in circular motions to aerate and help optimize bacterial growth
3. Visualizing: Microscopy, can't see with just the naked eye, must know parts of microscope
4. Storing: Refrigerate at 4 ° C, slows bacterial growth, prolongs life of sample at this
temperature, when not finished with experimentation
Lab Safety:
1. Never eat or drink in lab - contamination risks
2. Wear Personal Protective Equipment (PPE):
Use gloves: latex, nitrile (hypoallergenic) purple,blue or green, cold thermal - liquid
nitrogen, etc., heat thermal resistant gloves - extreme hot conditions
Eyewear protection - goggles
Lab coat - dependent on working with materials that may be spilt on clothes
3. NEVER leave lab while wearing PPE includes: Public Hallway, Restroom, Cafeteria
Lab Notebook:
First Name Initial, Last name initial, Experiment #, Title of Experiment
,BIOD 171 MICROLABS 1-9
Example: TK01 Keeping a Lab Notebook
Objective: Developing an organized lab notebook for documenting experiments, data,
procedures and records.
,BIOD 171 MICROLABS 1-9
Procedure:
1. Each step of lab protocol will be documented
2. Notate any clear deviations of lab experiment in red
Notes:
Additional information will be documented for future referencing.
Results:
Summary of final outcome of experiment, along with conclusions or the gist of the main
point from the experiment.
1. What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to
best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will
take several hours to achieve the same effect.
4. What type of incubator is pictured below?
Fixed incubator
, BIOD 171 MICROLABS 1-9
Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Objective, Procedure, Notes, Results and Deviations
Answer the following questions.
BIOD 171 MICROLABS 1-9
TK01 Keeping a Lab Notebook
Microbiology Lab1
Course Objectives:
Cultivation of samples (growth conditions) - equipment used
Identification of samples (biochemical assays) - tests available
Evaluation of samples (microscopy) - visualizations and recognitions key concepts
Basic Equipment:
1. Cleaning: autoclave 125 ° C, steamed, heat and pressure sterilization, chamber with thick
door, not dry air but steamed - more efficient, make sure depressurized before opening
Time restrictions: no autoclave then use steam (minutes) vs. hot air (hours)
2. Growing: fixed incubator = 37 ° C; shaker incubator = 37 ° C, for growth mediator, rotates and
shakes in circular motions to aerate and help optimize bacterial growth
3. Visualizing: Microscopy, can't see with just the naked eye, must know parts of microscope
4. Storing: Refrigerate at 4 ° C, slows bacterial growth, prolongs life of sample at this
temperature, when not finished with experimentation
Lab Safety:
1. Never eat or drink in lab - contamination risks
2. Wear Personal Protective Equipment (PPE):
Use gloves: latex, nitrile (hypoallergenic) purple,blue or green, cold thermal - liquid
nitrogen, etc., heat thermal resistant gloves - extreme hot conditions
Eyewear protection - goggles
Lab coat - dependent on working with materials that may be spilt on clothes
3. NEVER leave lab while wearing PPE includes: Public Hallway, Restroom, Cafeteria
Lab Notebook:
First Name Initial, Last name initial, Experiment #, Title of Experiment
,BIOD 171 MICROLABS 1-9
Example: TK01 Keeping a Lab Notebook
Objective: Developing an organized lab notebook for documenting experiments, data,
procedures and records.
,BIOD 171 MICROLABS 1-9
Procedure:
1. Each step of lab protocol will be documented
2. Notate any clear deviations of lab experiment in red
Notes:
Additional information will be documented for future referencing.
Results:
Summary of final outcome of experiment, along with conclusions or the gist of the main
point from the experiment.
1. What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to
best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will
take several hours to achieve the same effect.
4. What type of incubator is pictured below?
Fixed incubator
, BIOD 171 MICROLABS 1-9
Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Objective, Procedure, Notes, Results and Deviations
Answer the following questions.
