2018/9/11 Homework Week 2 (1): BIO SCI 99 LEC B: MOLECULAR BIOLOGY (05145)
Homework Week 2 (1)
Due Aug 21 at 11:59pm Points 11 Questions 11
Available after Aug 17 at 12pm Time Limit None Allowed Attempts 3
Attempt History
Attempt Time Score
KEPT Attempt 3 2 minutes 11 out of 11
LATEST Attempt 3 2 minutes 11 out of 11
Attempt 2 8 minutes 10 out of 11
Attempt 1 14 minutes 10 out of 11
Score for this attempt: 11 out of 11
Submitted Aug 21 at 10pm
This attempt took 2 minutes.
Question 1 pts
What is the sequence of the two primers that would be used in a PCR reaction with
the following strand of DNA as a template? The primers are 5 bases long and binds
to the end of the template. The answers below are
written in the 5' to 3' direction.
5' GACTGGACTGACATCGGA 3'
CTGAC
Correct! GACTG
Correct! TCCGA
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https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
,2018/9/11 Homework Week 2 (1): BIO SCI 99 LEC B: MOLECULAR BIOLOGY (05145)
None of the above
This study source was downloaded by 100000848398116 from CourseHero.com on 07-27-2022 01:06:18 GMT -05:00
https://ucirvine.instructure.com/courses/11150/quizzes/38536 2/9
https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
, 2018/9/11 Homework Week 2 (1): BIO SCI 99 LEC B: MOLECULAR BIOLOGY (05145)
Question 2 pts
You are setting up your PCR reaction and accidentally add twice as much of
the salt buffer as you were supposed to. Select all that apply.
How will this impact product formation?
In what way(s) will the reaction be altered?
Correct! ...because template denaturation will be altered.
You will get more of the desired PCR product...
Correct! You will get less of the desired PCR product...
...because the mechanism of dNTP addition will be altered.
You will get the same amount of the desired PCR product...
Correct! ...because primer/template binding will be altered.
Question 3 pts
You are setting up your PCR reaction and set the annealing temperature
lower than you were supposed to. What effect will this have?
You will not get PCR product.
Correct!
You will get PCR product, but multiple products, not only the expected product.
You will only get the expected PCR product.
Question 6 pts
This study source was downloaded by 100000848398116 from CourseHero.com on 07-27-2022 01:06:18 GMT -05:00
https://ucirvine.instructure.com/courses/11150/quizzes/38536 3/9
https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
Homework Week 2 (1)
Due Aug 21 at 11:59pm Points 11 Questions 11
Available after Aug 17 at 12pm Time Limit None Allowed Attempts 3
Attempt History
Attempt Time Score
KEPT Attempt 3 2 minutes 11 out of 11
LATEST Attempt 3 2 minutes 11 out of 11
Attempt 2 8 minutes 10 out of 11
Attempt 1 14 minutes 10 out of 11
Score for this attempt: 11 out of 11
Submitted Aug 21 at 10pm
This attempt took 2 minutes.
Question 1 pts
What is the sequence of the two primers that would be used in a PCR reaction with
the following strand of DNA as a template? The primers are 5 bases long and binds
to the end of the template. The answers below are
written in the 5' to 3' direction.
5' GACTGGACTGACATCGGA 3'
CTGAC
Correct! GACTG
Correct! TCCGA
https://ucirvine.instructure.com/courses/11150/quizzes/38536 1/9
https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
,2018/9/11 Homework Week 2 (1): BIO SCI 99 LEC B: MOLECULAR BIOLOGY (05145)
None of the above
This study source was downloaded by 100000848398116 from CourseHero.com on 07-27-2022 01:06:18 GMT -05:00
https://ucirvine.instructure.com/courses/11150/quizzes/38536 2/9
https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
, 2018/9/11 Homework Week 2 (1): BIO SCI 99 LEC B: MOLECULAR BIOLOGY (05145)
Question 2 pts
You are setting up your PCR reaction and accidentally add twice as much of
the salt buffer as you were supposed to. Select all that apply.
How will this impact product formation?
In what way(s) will the reaction be altered?
Correct! ...because template denaturation will be altered.
You will get more of the desired PCR product...
Correct! You will get less of the desired PCR product...
...because the mechanism of dNTP addition will be altered.
You will get the same amount of the desired PCR product...
Correct! ...because primer/template binding will be altered.
Question 3 pts
You are setting up your PCR reaction and set the annealing temperature
lower than you were supposed to. What effect will this have?
You will not get PCR product.
Correct!
You will get PCR product, but multiple products, not only the expected product.
You will only get the expected PCR product.
Question 6 pts
This study source was downloaded by 100000848398116 from CourseHero.com on 07-27-2022 01:06:18 GMT -05:00
https://ucirvine.instructure.com/courses/11150/quizzes/38536 3/9
https://www.coursehero.com/file/33054615/Homework-Week-2-1-BIO-SCI-99-LEC-B-MOLECULAR-BIOLOGY-05145pdf/
