BIOD 171 lab exams 1-9
Answer the following questions
1.What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2.What is the minimum temperature an autoclave must be set at to achieve
sterile condition?
125°C
3.If you are working in a lab in which an autoclave is not available, and you
are pressed for time, which would you chose to best sterilize your
equipment? Hot steam or hot air? Explain why you chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes
whereas hot air will take several hours to achieve the same effect.
4.What type of incubator is pictured below?
Fixed incubator
Page 1 of 28
,Answer Key
Page 2 of 28
, Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is
beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and
others from contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Answer the following questions.
1.You are a lab instructor and Sally Miller has turned in her lab notebook for
you to grade the lab experiment #1 on lab safety. Based upon what was
covered in the lab video, how should Sally have entitled her experiment?
SM01 Lab Safety
2.You arrive to your first day of work at a new lab. You are taking over for
someone who took a new job at another lab. Your boss informs you that
because of time restraints, this person did not exactly follow the
experimental protocol. In order to proceed, you must know what he did
differently. (1) According to the lab lecture, under what section of his
Page 3 of 28
Answer the following questions
1.What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2.What is the minimum temperature an autoclave must be set at to achieve
sterile condition?
125°C
3.If you are working in a lab in which an autoclave is not available, and you
are pressed for time, which would you chose to best sterilize your
equipment? Hot steam or hot air? Explain why you chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes
whereas hot air will take several hours to achieve the same effect.
4.What type of incubator is pictured below?
Fixed incubator
Page 1 of 28
,Answer Key
Page 2 of 28
, Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is
beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and
others from contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Answer the following questions.
1.You are a lab instructor and Sally Miller has turned in her lab notebook for
you to grade the lab experiment #1 on lab safety. Based upon what was
covered in the lab video, how should Sally have entitled her experiment?
SM01 Lab Safety
2.You arrive to your first day of work at a new lab. You are taking over for
someone who took a new job at another lab. Your boss informs you that
because of time restraints, this person did not exactly follow the
experimental protocol. In order to proceed, you must know what he did
differently. (1) According to the lab lecture, under what section of his
Page 3 of 28
