2
Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to
navigate): Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
1
,2
Lab 1 Notebook
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Title: KD01 Introduction to medical microbiology & lab notebook procedures
Course Objectives:
Cultivation of samples: growth conditions and equipment used
Identification of samples: biochemical assays & tests available
Evaluation of samples: microscopy- visualization and key characteristics of specimens
Basic Equipment:
Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take
minutes to sterilize & hot dry air which will take hours; before
opening make sure it is depressurized and will have very hot air.
Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes
- Shaker incubator 37°C shakes culture & rotates to
aerate- liquid nutrient broth
Visualizing: microscopy
Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term
Lab Safety
1. Never eat or drink in the lab: contamination risks
2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue,
green),thermal,
or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills
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3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria
Outline of how to set up Notebook
Objective: To establish an organized template for keeping experimental records, procedures,
and results.
Procedure:
1. This is where each step of a given protocol is recorded
2. Be sure to clearly indicate any deviations that may occur during the experiment-
put them in red
Notes: Additional (helpful) information placed here
Results: A summary of the final outcome of the experiment should be described here
3
, 2
Lab 2 Notebook
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Title: KD02 Introduction to Microscopy
Objective: To learn basic parts of microscope and how to view a sample
Procedure:
1. Review the parts of microscope
2. Load sample to be viewed
3. Choose magnification
4. Adjust microscope so the sample is clear
Notes:
Parts of microscope:
1. eye pieces (ocular lens): can be pulled apart; should be able to use both eyes and see
one circle if not adjust
2. arm/neck: if moving this is what you will grab with one hand and hand under base
3. Objective lenses: this provides the magnification of the sample; shortest has the
least magnification (4x, 10x, 40x,100x)
4. Stage: the flat surface that you place your sample; holds the sample via a clamp
(squeeze together to place sample and release); stage clips raise up and lay on the glass
coverslip
5. Focus knobs: located on the side of the arm/neck; Outside ring is the coarse adjustment
– makes large steps in focus; Inner ring is the fine focus- you can see the specimen but need
4
Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to
navigate): Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
1
,2
Lab 1 Notebook
Back to Home Page
Title: KD01 Introduction to medical microbiology & lab notebook procedures
Course Objectives:
Cultivation of samples: growth conditions and equipment used
Identification of samples: biochemical assays & tests available
Evaluation of samples: microscopy- visualization and key characteristics of specimens
Basic Equipment:
Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take
minutes to sterilize & hot dry air which will take hours; before
opening make sure it is depressurized and will have very hot air.
Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes
- Shaker incubator 37°C shakes culture & rotates to
aerate- liquid nutrient broth
Visualizing: microscopy
Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term
Lab Safety
1. Never eat or drink in the lab: contamination risks
2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue,
green),thermal,
or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills
2
,2
3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria
Outline of how to set up Notebook
Objective: To establish an organized template for keeping experimental records, procedures,
and results.
Procedure:
1. This is where each step of a given protocol is recorded
2. Be sure to clearly indicate any deviations that may occur during the experiment-
put them in red
Notes: Additional (helpful) information placed here
Results: A summary of the final outcome of the experiment should be described here
3
, 2
Lab 2 Notebook
Back to Home Page
Title: KD02 Introduction to Microscopy
Objective: To learn basic parts of microscope and how to view a sample
Procedure:
1. Review the parts of microscope
2. Load sample to be viewed
3. Choose magnification
4. Adjust microscope so the sample is clear
Notes:
Parts of microscope:
1. eye pieces (ocular lens): can be pulled apart; should be able to use both eyes and see
one circle if not adjust
2. arm/neck: if moving this is what you will grab with one hand and hand under base
3. Objective lenses: this provides the magnification of the sample; shortest has the
least magnification (4x, 10x, 40x,100x)
4. Stage: the flat surface that you place your sample; holds the sample via a clamp
(squeeze together to place sample and release); stage clips raise up and lay on the glass
coverslip
5. Focus knobs: located on the side of the arm/neck; Outside ring is the coarse adjustment
– makes large steps in focus; Inner ring is the fine focus- you can see the specimen but need
4
