Determination of serum Amylase
Amylase is an enzyme belonging to the class of hydro lases . It catalyzed
the break down of starch and glycogen starch consists of both amylose and
amylopectin . Amylose is along of glucose molecules linking by α (1 - 4)
glycosidic bonds and amylopectin is linked by α (1 - 6) linkages at the
branch points .
Amylase sources in the body :-
1. Pancreas and the Salivary glands are the major tissue sources of serum
amylase .
2. Skeletal muscle , small intestine and the fallopian tubes lesser
concentrations .
Digestion of starch begins in the mouth with the hydrolytic action of
Salivary , Salivary amylase activity pancreatic amylase then preform the
major digestive action of starch .
Types of amylase:
1- α-amylase
α-amylases names 1,4-α-D-glucan glucanohydrolase; glycogenase;
saccharogen amylase, it is breaks down long-chain carbohydrates
ultimately yielding maltotriose and maltose from amylose, or maltose,
glucose and "limit dextrin" from amylopectin.
2- β-Amylase
It names 1,4-α-D-glucan maltohydrolase; glycogenase;. β-amylase
catalyzes the hydrolysis of the second α-1,4 glycosidic bond, cleaving off
two glucose units (maltose) at a time.
3- γ-Amylase
1
Amylase is an enzyme belonging to the class of hydro lases . It catalyzed
the break down of starch and glycogen starch consists of both amylose and
amylopectin . Amylose is along of glucose molecules linking by α (1 - 4)
glycosidic bonds and amylopectin is linked by α (1 - 6) linkages at the
branch points .
Amylase sources in the body :-
1. Pancreas and the Salivary glands are the major tissue sources of serum
amylase .
2. Skeletal muscle , small intestine and the fallopian tubes lesser
concentrations .
Digestion of starch begins in the mouth with the hydrolytic action of
Salivary , Salivary amylase activity pancreatic amylase then preform the
major digestive action of starch .
Types of amylase:
1- α-amylase
α-amylases names 1,4-α-D-glucan glucanohydrolase; glycogenase;
saccharogen amylase, it is breaks down long-chain carbohydrates
ultimately yielding maltotriose and maltose from amylose, or maltose,
glucose and "limit dextrin" from amylopectin.
2- β-Amylase
It names 1,4-α-D-glucan maltohydrolase; glycogenase;. β-amylase
catalyzes the hydrolysis of the second α-1,4 glycosidic bond, cleaving off
two glucose units (maltose) at a time.
3- γ-Amylase
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