Name: Ysabel Mari M. Bisnan Professor: Ma’am Gecelene Esto
Date: June 23, 2022
Activity 4 Leaf Stomata
I. Introduction
Plants and animals both have a layer of tissue called the epidermal layer. This is the
layer of cells on the outside of the organism. Plants have special pores in there
epidermal cells. These pores are called stomata. The stomata allow the passage of
material in or out of the plant. The stomata pores are surrounded on both sides by
jellybean shaped cells called guard cells. The guard cells expand to open the stomata
and contract to close the stomata. When guard cells open carbon dioxide can enter the
plant leaf and oxygen can leave the plant. This allows the plant to perform
photosynthesis. Stomata is the plural of stomate Water vapor also can leave through a
stomate. Guard cells will close the stomata when the plant is dehydrated. This keeps
water in the plant from escaping and killing the plant. Most stomata are on the lower
epidermis of the leaves on plants. The lower epidermis is the bottom of the leaf. The
number of stomata on the epidermal surface can tell you a lot about a plant. Usually, a
high concentration of stomata indicates fast growth and wet climate. Lower
concentrations of stomata indicate lower rates of photosynthesis and growth or
adaptations for dry weather.
II. Objectives
To view and compare the stomata from the leaves of several species of plant
III. Materials
● 5 leaves (1 from 5 different species),
● Microscope
● Slides
● Clear nail polish
● Transparent tape
IV. Procedure
1. Obtain three leaves from different types of plants.
2. Paint a thick patch (at least one square centimeter) of clear nail polish on the underside of th
studied.
3. Allow the nail polish to dry completely.
4. Tape a piece of clear tape to the dried nail polish patch.
5. Gently peel the nail polish patch from the leaf by pulling on a corner of the tape and "peeling"
off the leaf. This is the leaf impression you will examine.
6. Tape your peeled impression to a very clean microscope slide. Use scissors to trim away any
slide with plant name.
7. Examine the leaf impression under a light microscope.
8. Search for areas where there are numerous stomata, and where there are no dirt, thumb prin
large leaf veins. Draw the leaf surface with stomata.
9. Count all the stomata in one microscopic field. Record the number on your data table.
10. Repeat counts for at least three other distinct microscopic fields. Record all the counts. Dete
an average number per microscopic field.
11. From the average number microscopic field, calculate the stomata per mm2 by multiplying
12. Follow procedures 2 - 11 with the other leaves.
V. Schematic Diagram:
Date: June 23, 2022
Activity 4 Leaf Stomata
I. Introduction
Plants and animals both have a layer of tissue called the epidermal layer. This is the
layer of cells on the outside of the organism. Plants have special pores in there
epidermal cells. These pores are called stomata. The stomata allow the passage of
material in or out of the plant. The stomata pores are surrounded on both sides by
jellybean shaped cells called guard cells. The guard cells expand to open the stomata
and contract to close the stomata. When guard cells open carbon dioxide can enter the
plant leaf and oxygen can leave the plant. This allows the plant to perform
photosynthesis. Stomata is the plural of stomate Water vapor also can leave through a
stomate. Guard cells will close the stomata when the plant is dehydrated. This keeps
water in the plant from escaping and killing the plant. Most stomata are on the lower
epidermis of the leaves on plants. The lower epidermis is the bottom of the leaf. The
number of stomata on the epidermal surface can tell you a lot about a plant. Usually, a
high concentration of stomata indicates fast growth and wet climate. Lower
concentrations of stomata indicate lower rates of photosynthesis and growth or
adaptations for dry weather.
II. Objectives
To view and compare the stomata from the leaves of several species of plant
III. Materials
● 5 leaves (1 from 5 different species),
● Microscope
● Slides
● Clear nail polish
● Transparent tape
IV. Procedure
1. Obtain three leaves from different types of plants.
2. Paint a thick patch (at least one square centimeter) of clear nail polish on the underside of th
studied.
3. Allow the nail polish to dry completely.
4. Tape a piece of clear tape to the dried nail polish patch.
5. Gently peel the nail polish patch from the leaf by pulling on a corner of the tape and "peeling"
off the leaf. This is the leaf impression you will examine.
6. Tape your peeled impression to a very clean microscope slide. Use scissors to trim away any
slide with plant name.
7. Examine the leaf impression under a light microscope.
8. Search for areas where there are numerous stomata, and where there are no dirt, thumb prin
large leaf veins. Draw the leaf surface with stomata.
9. Count all the stomata in one microscopic field. Record the number on your data table.
10. Repeat counts for at least three other distinct microscopic fields. Record all the counts. Dete
an average number per microscopic field.
11. From the average number microscopic field, calculate the stomata per mm2 by multiplying
12. Follow procedures 2 - 11 with the other leaves.
V. Schematic Diagram:
