Name: Bisnan, Ysabel Mari M. Professor: Ma’am Gecelene
Date: June 8, 2022
Activity 2 Plasmolysis
I. Introduction
Plasmolysis Definition
Plasmolysis is when plant cells lose water after being placed in a solution that has a higher concentra
cell does. This is known as a hypertonic solution. Water flows out of the cells and into the surroundi
This causes the protoplasm, all the material on the inside of the cell, to shrink away from the cell wal
leads to the collapse of the cell wall can result in cell death. Since osmosis is a process that requires n
the cell and cannot be controlled, cells cannot stop plasmolysis from taking place.
Plasmolysis and Osmosis
Osmosis is responsible for the occurrence of plasmolysis. Osmosis is a special type of diffusion that o
into or out of a membrane such as a cell’s plasma membrane. It occurs based on the type of solution th
is a mixture that contains a fluid, or solvent (usually water), and a solute that is dissolved in the solve
into a hypertonic solution, there is a higher concentration of solutes outside the cell, so water flows o
the concentration on both sides of the membrane. Since plasmolysis is the loss of water from a cell, it o
hypertonic solution. Conversely, when a cell is placed into a hypotonic solution, there is a lower solut
the cell than inside, and water rushes into the cell. In an isotonic solution, solute concentrations are th
there is no net gain or loss of water.
I. Objectives
● This experiment aims to study the phenomenon of plasmolysis, which takes place in plant cells.
II. Materials
- Sodium Chloride Solution 0.65%
- Distilled water
- Glass slides
- Cover slip
- Laser lens
- Flashlight
- Cellphone
- Double sided tape
- Dropper
- Rhoeo leaf
III. Procedure
1. Prepare the materials needed
2. Dilute 0.65% Sodium chloride with 5.5 mL distilled water to produce 0.1% concentration
3. Dilute 0.65% Sodium chloride with 3.5 mL distilled water to produce 0.3% concentration
4. Remove a small portion of the violet part of the rhoeo leaf and put it on the glass slide
5. Add few drops of the 0.1, 0.3, and 0.65% solution on each slide and put the cover slip
6. Wait for 30 minutes
Date: June 8, 2022
Activity 2 Plasmolysis
I. Introduction
Plasmolysis Definition
Plasmolysis is when plant cells lose water after being placed in a solution that has a higher concentra
cell does. This is known as a hypertonic solution. Water flows out of the cells and into the surroundi
This causes the protoplasm, all the material on the inside of the cell, to shrink away from the cell wal
leads to the collapse of the cell wall can result in cell death. Since osmosis is a process that requires n
the cell and cannot be controlled, cells cannot stop plasmolysis from taking place.
Plasmolysis and Osmosis
Osmosis is responsible for the occurrence of plasmolysis. Osmosis is a special type of diffusion that o
into or out of a membrane such as a cell’s plasma membrane. It occurs based on the type of solution th
is a mixture that contains a fluid, or solvent (usually water), and a solute that is dissolved in the solve
into a hypertonic solution, there is a higher concentration of solutes outside the cell, so water flows o
the concentration on both sides of the membrane. Since plasmolysis is the loss of water from a cell, it o
hypertonic solution. Conversely, when a cell is placed into a hypotonic solution, there is a lower solut
the cell than inside, and water rushes into the cell. In an isotonic solution, solute concentrations are th
there is no net gain or loss of water.
I. Objectives
● This experiment aims to study the phenomenon of plasmolysis, which takes place in plant cells.
II. Materials
- Sodium Chloride Solution 0.65%
- Distilled water
- Glass slides
- Cover slip
- Laser lens
- Flashlight
- Cellphone
- Double sided tape
- Dropper
- Rhoeo leaf
III. Procedure
1. Prepare the materials needed
2. Dilute 0.65% Sodium chloride with 5.5 mL distilled water to produce 0.1% concentration
3. Dilute 0.65% Sodium chloride with 3.5 mL distilled water to produce 0.3% concentration
4. Remove a small portion of the violet part of the rhoeo leaf and put it on the glass slide
5. Add few drops of the 0.1, 0.3, and 0.65% solution on each slide and put the cover slip
6. Wait for 30 minutes
