DATE: 20/08/2019
EXPERIMENT NO: 2
BIOLOGICAL CHARACTERS OF
ENTEROBACTERIACEAE
Identification of enteric bacilli is of prime important in controlling intestinal
infections by preventing contamination of food and water supplies. The group of
bacteria that can be by preventing in intestinal tract of humans and lower
mammals are classifies as member of the family enterobacteriaceae. They are
gram negative and non-sporing bacilli.
Included in this family are Shigella, Salmonella, Protease, Klebsiella, E coli,
Enterobacter etc. The four tests indole, methyl red, Voges Proskaur, Citrate
collectively called IMViC series of reaction. The IMViC series of reaction allows
for the differentiation of the various members of enterobacteriacea.
SCIENTIFIC CLASSIFICATION:
Domain-Bacteria
Phylum-Proteobacteria
Class-Gamma protobacteria
Order- Enterobacterial
Family-Enterobacteriaceae
CHARACTERSTICS
Members of the Enterobacteriaceae are rod shaped. Typically 1 to 5
micrometers in length and are Gram negative, nonsporing. Some genera
are motile by means of peritrichous flagella except Tatunella, Shigella and
Klebsiella species which are non-motile. They are facultative anaerobic
and most species grow at 37 °C, although some species grow better at 25 to
30 °C. They grow well on peptone and meat extract media. Some strains
grow on D-glucose and other carbohydrates. They are reduced to nitrites
except by some strains of Erwinia. They are distributed worldwide and
may be found in soil, water, plants, humans and animals. Some
Enterbacteria produce endotoxins. Endotoxins reside in the cell wall and
are released into blood stream following cell lysis, causing a systemic
inflammatory and vasodilatory response known as endotoxin shock. Cell
EXPERIMENT NO: 2
BIOLOGICAL CHARACTERS OF
ENTEROBACTERIACEAE
Identification of enteric bacilli is of prime important in controlling intestinal
infections by preventing contamination of food and water supplies. The group of
bacteria that can be by preventing in intestinal tract of humans and lower
mammals are classifies as member of the family enterobacteriaceae. They are
gram negative and non-sporing bacilli.
Included in this family are Shigella, Salmonella, Protease, Klebsiella, E coli,
Enterobacter etc. The four tests indole, methyl red, Voges Proskaur, Citrate
collectively called IMViC series of reaction. The IMViC series of reaction allows
for the differentiation of the various members of enterobacteriacea.
SCIENTIFIC CLASSIFICATION:
Domain-Bacteria
Phylum-Proteobacteria
Class-Gamma protobacteria
Order- Enterobacterial
Family-Enterobacteriaceae
CHARACTERSTICS
Members of the Enterobacteriaceae are rod shaped. Typically 1 to 5
micrometers in length and are Gram negative, nonsporing. Some genera
are motile by means of peritrichous flagella except Tatunella, Shigella and
Klebsiella species which are non-motile. They are facultative anaerobic
and most species grow at 37 °C, although some species grow better at 25 to
30 °C. They grow well on peptone and meat extract media. Some strains
grow on D-glucose and other carbohydrates. They are reduced to nitrites
except by some strains of Erwinia. They are distributed worldwide and
may be found in soil, water, plants, humans and animals. Some
Enterbacteria produce endotoxins. Endotoxins reside in the cell wall and
are released into blood stream following cell lysis, causing a systemic
inflammatory and vasodilatory response known as endotoxin shock. Cell
