BOC Fixation(Questions and Answers 100% correct.

BOC Fixation(Questions and Answers 100% correct. An example of an additive fixative is one that contains: A. Picric Acid B. Acetic Acid C. Ethyl Alcohol D. Acetone Picric Acid When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: A. Better ultrastructural preservation B. Decreased immunoreactivity C. Increased enzyme activity D. Superior nuclear detail Superior nuclear detail 00:21 01:08 Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: A. Incomplete fixation B. Prolonged fixation C. Microwave fixation D. Frozen Sectioning Incomplete fixation Microscopic evaluation of H&E stained sections from a surgically removed small bowel specimen shows an absence of much of the epithelium in otherwise normal tissue. This is most likely resulted from: A. Mechanical trauma B. Delayed fixation C. Ulceration D. Poor choice of fixative Delayed fixation A specimen of kidney must be shipped to another city for immunofluorescence studies. The specimen should be placed in : A. Saline B. Michel solution C. Buffered formalin D. Orth Michel solution A certain project requires a fixative that contains acetic acid yet stabilizes erythrocyte membranes. One fixative that could be used is: A. Zenker B. Bouin C. Gendre D. Hollande Hollande When the microwave oven is used for fixation, the most critical factor is the: A. Preparation of the formalin solution B. Use of glass containers C. Control of the temp D. Osmolality of the fixation solution Control of the temp To adequately remove the calcium from a specimen containing areas of micro-calcification, the tissue could be fixed in: A. Hollande B. NBF C. B-5 D. Zamboni Hollande A specimen is submitted with the statement that it was fixed in formalin. Microscopic sections show marked lysis of erythrocytes. This indicates that the fixative most likely was: A. Prepared with too much formalin B. Buffered above neutrality C. Acidified with acetic acid D. Not formalin Acidified with acetic acid Fixatives are classified as additive because of the: A. Addition of several chemicals to the solution B. Addition, or binding of the fixative to tissue proteins C. Additional reactions occurring with longer fixation D. Additional reactive tissue sites available for dye binding Addition, or binding of the fixative to tissue proteins Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde for 2 hours and then placed in phosphate buffer solution. If a portion of this tissue is processed for light microscopy, sections would most likely show: A. Very poor glomerular preservation B. Decreased uptake of hematoxylin C. Lysis of cytoplasmic elements D. Nonspecific PAS staining Nonspecific PAS staining Uric acid crystals are preserved ONLY when tissue is fixed in: A. Absolute alcohol B. NBF C. Orth D. Zamboni Absolute alcohol 00:00 Upgrade to remove ads Only $35.99/year Improper preservation of tissue will result if there is: A. A delay in fixation B. Rapid penetration of the fixing fluid C. Prolonged storage following formalin fixation D. Rapid dehydration, clearing, embedding, ad sectioning A delay in fixation A good fixative will: A. Render cell constituents soluble B. Minimize differences in tissue refractive indices C. Protect tissue against alteration during subsequent processing D. Minimally affect tissue metabolic processes Protect tissue against alteration during subsequent processing The function of methanol in commercial formalin solutions is to: A. Retard the polymerization of formaldehyde B. Prevent the formation of formic acid C. Stabilize the formalin at a basic pH D. Permit room temperature storage of formalin Retard the polymerization of formaldehyde Which of the following fixatives contains copper acetate? A. Hollande B. Bouin C. Gendre D. Zamboni Hollande In electron microscopy, Zamboni fluid, glutaraldehyde, and osmium tetroxide function as: A. Dehydrating agents B. Clearing agents C. Embedding media D. Fixative solutions Fixative solutions Tissue will remain unfixed if placed in: A. Potassium dichromate B. Sodium borate C. Osmium tetroxide D. Zinc chloride Sodium borate Bouin solution is contraindicated for: A. Small tissue biopsies B. Tissue intended for subsequent trichrome stains C. Tissue to be stained by the Feulgen reaction D. Routine tissue sections Tissue to be stained by the Feulgen reaction Formalin pigment can be removed from tissue sections by treatment with 10% A. Hydrochloric acid in 70% alcohol B. Nitric acid in 70% alcohol C. Sulfuric acid in 70% alcohol D. Ammonium hydroxide in 70% alcohol Ammonium hydroxide in 70% alcohol Stock neutralized formalin is prepared in the lab by storing the solution over a layer of calcium carbonate. The solution withdrawn from this stock container will: A. Become acidic B. Become alkaline C. Remain neutral D. Exhibit metachromasia Become acidic Microscopic evaluation reveals a poorly stained H&E section of spleen. These results will be difficult to remedy if the problem is: A. Poor fixation B. Improper sectioning C. Poor staining D. Incorrect section placement Poor fixation To make a 10% formalin solution, how many mL of water should be added to 300 mL of 37-40% formaldehyde solution? A. 1,800 B. 2,500 C. 2,700 D. 3,600 2,700 One action of acetic acid is to: A. Exert a shrinking effect on tissue B. Render nucleoprotein acidophilic C. Form salt linkages between protein chains D. Coagulate nucleoproteins Coagulate nucleoproteins Aldehyde fixatives are used for electron microscopy preparations because they: A. Are readily available B. Visibly stain tissue C. Preserve cell ultrastructure D. Coagulate tissue lipids Preserve cell ultrastructure