Cell Biology/Cell Biochemistry: Final Exam-‐-‐-‐10AM class
Choose the single best answer
1) Which of the following choices best describes the role of the lysosome?
(a) transport of material to the Golgi
(b) clean-up, recycling, and disposal of macromolecules
(c) sorting of transport vesicles
(d) the storage of excess macromolecules
2) are fairly small organelles that provide a safe place within the cell to carry out
certain biochemical reactions that generate harmful, highly reactive oxygen species. These
chemicals are both generated and broken down in the same location.
(a) Nucleosomes
(b) Lysosomes
(c) Peroxisomes
(d) Endosomes
3) Which of the following monomer building blocks is necessary to assemble selectively
permeable boundaries around and inside cells?
(a) sugars
(b) fatty acids
(c) amino acids
(d) nucleotides
4) DNA and RNA are different types of nucleic acid polymer. Which of the following is true of DNA
but not true of RNA?
(a) It contains uracil.
(b) It contains thymine.
(c) It is single-stranded.
(d) It has 5′-to-3′ directionality.
5) Because there are four different monomer building blocks that can be used to assemble RNA
polymers, the number of possible sequence combinations that can be created for an RNA
molecule made of 100 nucleotides is .
(a) 1004
(b) 4100
(c) 4 × 100
(d) 100/4
,6) Macromolecules in the cell can often interact transiently as a result of noncovalent interactions.
These weak interactions also produce stable, highly specific interactions between molecules.
Which of the factors below is the most significant in determining whether the interaction will
be transient or stable?
(a) the size of each molecule
(b) the concentration of each molecule
(c) the rate of synthesis
(d) surface complementarity between molecules
7) The first task you are assigned in your summer laboratory job is to prepare a concentrated
NaOH stock solution. The molecular weight of NaOH is 40. How many grams of solid NaOH will
you need to weigh out to obtain a 500 mL solution that has a concentration of 10 M?
(a) 800 g
(b) 200 g
(c) 400 g
(d) 160 g
8) Oxidation is the process by which oxygen atoms are added to a target molecule. Generally, the
atom that is oxidized will experience which of the following with respect to the electrons in its
outer shell?
(a) a net gain
(b) a net loss
(c) no change
(d) an equal sharing
9) The potential energy stored in high-‐energy bonds is commonly harnessed when the bonds are
split by the addition of in a process called .
(a) ATP, phosphorylation.
(b) water, hydrolysis.
(c) hydroxide, hydration.
(d) acetate, acetylation.
10) The maximum velocity (Vmax) of an enzymatic reaction is an important piece of information
regarding how the enzyme works. What series of measurements can be taken in order to infer
the maximum velocity of an enzyme-‐catalyzed reaction?
(a) the rate of substrate consumption after the system reaches equilibrium, for several reactant
concentrations
(b) the rate of product consumption shortly after mixing the enzyme and substrate
(c) the rate of substrate consumption at high levels of enzyme concentration
(d) the rate of substrate consumption shortly after mixing the enzyme and substrate, for several
substrate concentrations
, 11) The synthesis of glutamine from glutamic acid requires the production of an activated
intermediate followed by a condensation step that completes the process. Both amino acids are
shown in the figure below.
In the condensation step, is displaced by .
(a) OH, NH3.
(b) ADP, NH2.
(c) ATP, NH3.
(d) phosphate, NH3.
12) Which of the following statements is true?
(a) Peptide bonds are the only covalent bonds that can link together two amino acids in proteins.
(b) The polypeptide backbone is free to rotate about each peptide bond.
(c) Nonpolar amino acids tend to be found in the interior of proteins.
(d) The sequence of the atoms in the polypeptide backbone varies between different proteins.
13) Which of the following is not true of molecular chaperones?
(a) They assist polypeptide folding by helping the folding process follow the most energetically
favorable pathway.
(b) They can isolate proteins from other components of the cells until folding is complete.
(c) They can interact with unfolded polypeptides in a way that changes the final fold of the
protein.
(d) They help streamline the protein-folding process by making it a more efficient and reliable
process inside the cell.
14) Coiled-‐coils are typically found in proteins that require an elongated structural framework.
Which of the following proteins do you expect to have a coiled-‐coil domain?
(a) insulin
(b) collagen
(c) myoglobin
(d) porin
15) Which DNA base pair is represented in the figure below?
(a) A-T
(b) T-A
(c) G-C
(d) C-G
Choose the single best answer
1) Which of the following choices best describes the role of the lysosome?
(a) transport of material to the Golgi
(b) clean-up, recycling, and disposal of macromolecules
(c) sorting of transport vesicles
(d) the storage of excess macromolecules
2) are fairly small organelles that provide a safe place within the cell to carry out
certain biochemical reactions that generate harmful, highly reactive oxygen species. These
chemicals are both generated and broken down in the same location.
(a) Nucleosomes
(b) Lysosomes
(c) Peroxisomes
(d) Endosomes
3) Which of the following monomer building blocks is necessary to assemble selectively
permeable boundaries around and inside cells?
(a) sugars
(b) fatty acids
(c) amino acids
(d) nucleotides
4) DNA and RNA are different types of nucleic acid polymer. Which of the following is true of DNA
but not true of RNA?
(a) It contains uracil.
(b) It contains thymine.
(c) It is single-stranded.
(d) It has 5′-to-3′ directionality.
5) Because there are four different monomer building blocks that can be used to assemble RNA
polymers, the number of possible sequence combinations that can be created for an RNA
molecule made of 100 nucleotides is .
(a) 1004
(b) 4100
(c) 4 × 100
(d) 100/4
,6) Macromolecules in the cell can often interact transiently as a result of noncovalent interactions.
These weak interactions also produce stable, highly specific interactions between molecules.
Which of the factors below is the most significant in determining whether the interaction will
be transient or stable?
(a) the size of each molecule
(b) the concentration of each molecule
(c) the rate of synthesis
(d) surface complementarity between molecules
7) The first task you are assigned in your summer laboratory job is to prepare a concentrated
NaOH stock solution. The molecular weight of NaOH is 40. How many grams of solid NaOH will
you need to weigh out to obtain a 500 mL solution that has a concentration of 10 M?
(a) 800 g
(b) 200 g
(c) 400 g
(d) 160 g
8) Oxidation is the process by which oxygen atoms are added to a target molecule. Generally, the
atom that is oxidized will experience which of the following with respect to the electrons in its
outer shell?
(a) a net gain
(b) a net loss
(c) no change
(d) an equal sharing
9) The potential energy stored in high-‐energy bonds is commonly harnessed when the bonds are
split by the addition of in a process called .
(a) ATP, phosphorylation.
(b) water, hydrolysis.
(c) hydroxide, hydration.
(d) acetate, acetylation.
10) The maximum velocity (Vmax) of an enzymatic reaction is an important piece of information
regarding how the enzyme works. What series of measurements can be taken in order to infer
the maximum velocity of an enzyme-‐catalyzed reaction?
(a) the rate of substrate consumption after the system reaches equilibrium, for several reactant
concentrations
(b) the rate of product consumption shortly after mixing the enzyme and substrate
(c) the rate of substrate consumption at high levels of enzyme concentration
(d) the rate of substrate consumption shortly after mixing the enzyme and substrate, for several
substrate concentrations
, 11) The synthesis of glutamine from glutamic acid requires the production of an activated
intermediate followed by a condensation step that completes the process. Both amino acids are
shown in the figure below.
In the condensation step, is displaced by .
(a) OH, NH3.
(b) ADP, NH2.
(c) ATP, NH3.
(d) phosphate, NH3.
12) Which of the following statements is true?
(a) Peptide bonds are the only covalent bonds that can link together two amino acids in proteins.
(b) The polypeptide backbone is free to rotate about each peptide bond.
(c) Nonpolar amino acids tend to be found in the interior of proteins.
(d) The sequence of the atoms in the polypeptide backbone varies between different proteins.
13) Which of the following is not true of molecular chaperones?
(a) They assist polypeptide folding by helping the folding process follow the most energetically
favorable pathway.
(b) They can isolate proteins from other components of the cells until folding is complete.
(c) They can interact with unfolded polypeptides in a way that changes the final fold of the
protein.
(d) They help streamline the protein-folding process by making it a more efficient and reliable
process inside the cell.
14) Coiled-‐coils are typically found in proteins that require an elongated structural framework.
Which of the following proteins do you expect to have a coiled-‐coil domain?
(a) insulin
(b) collagen
(c) myoglobin
(d) porin
15) Which DNA base pair is represented in the figure below?
(a) A-T
(b) T-A
(c) G-C
(d) C-G
