Summary Molisch's test

Molisch's test

Molisch Test Definition
Molisch test is a group test for
all carbohydrates, either free or
bound to proteins or lipids. It is a
sensitive test that requires precision
for the detection of carbohydrates.
Objectives of Molisch Test

• To detect the presence of
carbohydrates in a given
sample.


• To distinguish carbohydrates
from other biomolecules.


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, Principle of Molisch Test




• The reaction is based on the
fact that the concentrated acid
catalyzes the dehydration of
sugars to form furfural (from
pentoses) or
hydroxymethylfurfural (from
hexoses).

• Either of these aldehydes
condenses with two molecules
of naphthol to form a purple or
violet colored complex at the


2