Summary Liver Function Test .Detailed notes.

 USED TO  Detect presence of liver disease  Distinguish among different types of liver ds  Gauge the extent of known liver damage  Follow the response of treatment  Rarely suggest a specific diagnosis functions  Serum bilirubin  Urine bilirubin  Blood ammonia  Serum enzymes : AST, ALT, GGT, 5’Nucleotidase,ALP of liver  Serum Albumin  Serum Globulins  PT ,INR  A break down product of porphyrin ring of heme – containing proteins , found in blood in 2 fractions – conj/unconj  Conjugated : water soluble , so excreted by kidneys  Unconjugated : insoluble in water , bound to albumin in blood  About 300 mg of bilirubin is formed per day  Normal total serum bilirubin: 0.3 – 1.3 mg/dl  Direct/conjugated bilirubin: 0.1 – 0.4 mg/dl  Indirect/unconjugated bilirubin: 0.2 – 0.9mg/dl  Measured by Van Den Bergh method  Bilirubin reacts with diazo reagent to produce coloured azo pigment . At pH 5 – pigment purple .