DNA Sequencing
By
HASNAT MUEEN
, Table of Contents
1 Introduction ........................................................................................................................ 1
2 Advantages and Applications ............................................................................................. 1
3 Basic Steps of DNA Sequencing ........................................................................................ 2
4 DNA Sequencing Methods ................................................................................................. 2
4.1 First Generation DNA Sequencing ............................................................................. 2
4.1.1 Chemical Degradation Method ............................................................................ 3
4.1.2 Chain Termination Method .................................................................................. 4
4.1.3 Modification in 1st Generation Methods .............................................................. 5
4.2 Next Generation DNA Sequencing ............................................................................. 5
4.2.1 Salient Features of NGS....................................................................................... 6
4.2.2 NGS Methods....................................................................................................... 6
4.3 Third Generation DNA sequencing............................................................................. 7
4.3.1 TGS methods ....................................................................................................... 8
By
HASNAT MUEEN
, Table of Contents
1 Introduction ........................................................................................................................ 1
2 Advantages and Applications ............................................................................................. 1
3 Basic Steps of DNA Sequencing ........................................................................................ 2
4 DNA Sequencing Methods ................................................................................................. 2
4.1 First Generation DNA Sequencing ............................................................................. 2
4.1.1 Chemical Degradation Method ............................................................................ 3
4.1.2 Chain Termination Method .................................................................................. 4
4.1.3 Modification in 1st Generation Methods .............................................................. 5
4.2 Next Generation DNA Sequencing ............................................................................. 5
4.2.1 Salient Features of NGS....................................................................................... 6
4.2.2 NGS Methods....................................................................................................... 6
4.3 Third Generation DNA sequencing............................................................................. 7
4.3.1 TGS methods ....................................................................................................... 8
