Next Generation Sequencing
By
HASNAT MUEEN
, Table of Contents
1 Introduction ........................................................................................................................ 1
2 Salient Features of NGS ..................................................................................................... 1
3 Preparation of NGS Library ............................................................................................... 2
3.1 DNA Fragmentation .................................................................................................... 2
3.2 Immobilization ............................................................................................................ 2
3.3 Amplification .............................................................................................................. 3
4 NGS Methods ..................................................................................................................... 3
4.1 Massively Parallel Signature Sequencing (MPSS) ..................................................... 4
4.2 Polony Sequencing ...................................................................................................... 4
4.3 Pyrosequencing ........................................................................................................... 4
4.4 Illumina Sequencing .................................................................................................... 5
4.5 SOLiD Sequencing ...................................................................................................... 5
4.6 Ion Torrent Sequencing ............................................................................................... 6
4.7 DNA Nano-ball Sequencing........................................................................................ 6
4.8 Helioscope single-molecule sequencing ..................................................................... 7
5 Applications of NGS .......................................................................................................... 7
By
HASNAT MUEEN
, Table of Contents
1 Introduction ........................................................................................................................ 1
2 Salient Features of NGS ..................................................................................................... 1
3 Preparation of NGS Library ............................................................................................... 2
3.1 DNA Fragmentation .................................................................................................... 2
3.2 Immobilization ............................................................................................................ 2
3.3 Amplification .............................................................................................................. 3
4 NGS Methods ..................................................................................................................... 3
4.1 Massively Parallel Signature Sequencing (MPSS) ..................................................... 4
4.2 Polony Sequencing ...................................................................................................... 4
4.3 Pyrosequencing ........................................................................................................... 4
4.4 Illumina Sequencing .................................................................................................... 5
4.5 SOLiD Sequencing ...................................................................................................... 5
4.6 Ion Torrent Sequencing ............................................................................................... 6
4.7 DNA Nano-ball Sequencing........................................................................................ 6
4.8 Helioscope single-molecule sequencing ..................................................................... 7
5 Applications of NGS .......................................................................................................... 7
