Special Culture requirements
Bug Media used for isolation
H. influenza Chocolate agar with factors V(NAD) & X (hematin)
N. Gonorrhoeae Thayer-Martin media
B.Pertussis Bordet-Gengau (potato) agar
C.diphteriae Tellurite plate, loffler’s media
M.tuberculosis Lowenstein-Jensen agar
Lactose fermenting enterics Pink colonies on MacConkey agar
Legionella Charcoal yeast extract agar buffered wth increase iron & cysteine
Fungi Sabouraud’s agar
Stains
Congo red – Amyloid; apple green birefringence in polarized light (bcoz of b-pleated sheet)
Giemsa’s – H.pylori, Plasmodium, Chlamydia,
PAS (periodic acid-Schiff) – stains glycogen, mucopolysacch; used to dx whipple’s dz
Ziehl-Nielsen – Acid-fast bacteria
India ink – Cryptococcus neoformans
Silver stain – Fungi, legionella
Oil red o - fat
Bug Media used for isolation
H. influenza Chocolate agar with factors V(NAD) & X (hematin)
N. Gonorrhoeae Thayer-Martin media
B.Pertussis Bordet-Gengau (potato) agar
C.diphteriae Tellurite plate, loffler’s media
M.tuberculosis Lowenstein-Jensen agar
Lactose fermenting enterics Pink colonies on MacConkey agar
Legionella Charcoal yeast extract agar buffered wth increase iron & cysteine
Fungi Sabouraud’s agar
Stains
Congo red – Amyloid; apple green birefringence in polarized light (bcoz of b-pleated sheet)
Giemsa’s – H.pylori, Plasmodium, Chlamydia,
PAS (periodic acid-Schiff) – stains glycogen, mucopolysacch; used to dx whipple’s dz
Ziehl-Nielsen – Acid-fast bacteria
India ink – Cryptococcus neoformans
Silver stain – Fungi, legionella
Oil red o - fat
