OpenStax Microbiology Test Bank
Chapter 12: Modern Applications of
Microbial Genetics
Page 1 of 20
,Chapter 12: Modern Applications of Microbial Genetics
* = Correct answer
Multiple Choice
1. Which of the following is the definition of biotechnology?
A. The alteration of DNA in a cell
B. The use of living systems to benefit humankind*
C. Using computers to synthesize biomolecules
D. Using DNA for solving mathematical problems
Difficulty: Easy
ASM Standard: 26
2. Which of the following is the definition of genetic engineering?
A. The alteration of DNA in a cell*
B. The use of living systems to benefit humankind
C. Using computers to synthesize biomolecules
D. Using DNA for solving mathematical problems
Difficulty: Easy
ASM Standard: 15, 19, 26, 31
3. The first set of genes to be introduced into E. coli came from which of the following?
A. Arabidopsis
B. Haemophilus
C. Mycobacterium
D. Xenopus*
Difficulty: Easy
ASM Standard: 15, 19
Page 2 of 20
, 4. Which of the following is a palindrome sequence that could be recognized by a restriction
enzyme?
A. 5′ – GAAAAG – 3′
3′ – CTTTTC – 5′
B. 5′ – GAATTC – 3′ *
3′ – CTTAAG – 5′
C. 5′ – GACT – 3′
3′ – CTGA – 5′
D. 5′ – GCCCC – 3′
3′ – CGGGG – 5′
Difficulty: Moderate
ASM Standard: 26, 36
5. Restriction enzymes (endonucleases) are produced by bacteria as a means of protection
against which infectious agent?
A. antibiotics
B. antibodies
C. bacteriophages*
D. prions
Difficulty: Easy
ASM Standard: 18, 26
6. Which of the following enzymes rejoins two sugar -phosphate backbones of DNA?
A. helicase
B. ligase*
C. restriction enzyme
D. reverse transcriptase
Difficulty: Easy
ASM Standard: 26, 36
7. Genes to be cloned into a plasmid are typically inserted in which location?
A. in a location disrupting the antibiotic selective marker
B. oriC (origin of replication)
C. polylinker site*
D. randomly
Difficulty: Easy
ASM Standard: 19, 26, 36
Page 3 of 20
Chapter 12: Modern Applications of
Microbial Genetics
Page 1 of 20
,Chapter 12: Modern Applications of Microbial Genetics
* = Correct answer
Multiple Choice
1. Which of the following is the definition of biotechnology?
A. The alteration of DNA in a cell
B. The use of living systems to benefit humankind*
C. Using computers to synthesize biomolecules
D. Using DNA for solving mathematical problems
Difficulty: Easy
ASM Standard: 26
2. Which of the following is the definition of genetic engineering?
A. The alteration of DNA in a cell*
B. The use of living systems to benefit humankind
C. Using computers to synthesize biomolecules
D. Using DNA for solving mathematical problems
Difficulty: Easy
ASM Standard: 15, 19, 26, 31
3. The first set of genes to be introduced into E. coli came from which of the following?
A. Arabidopsis
B. Haemophilus
C. Mycobacterium
D. Xenopus*
Difficulty: Easy
ASM Standard: 15, 19
Page 2 of 20
, 4. Which of the following is a palindrome sequence that could be recognized by a restriction
enzyme?
A. 5′ – GAAAAG – 3′
3′ – CTTTTC – 5′
B. 5′ – GAATTC – 3′ *
3′ – CTTAAG – 5′
C. 5′ – GACT – 3′
3′ – CTGA – 5′
D. 5′ – GCCCC – 3′
3′ – CGGGG – 5′
Difficulty: Moderate
ASM Standard: 26, 36
5. Restriction enzymes (endonucleases) are produced by bacteria as a means of protection
against which infectious agent?
A. antibiotics
B. antibodies
C. bacteriophages*
D. prions
Difficulty: Easy
ASM Standard: 18, 26
6. Which of the following enzymes rejoins two sugar -phosphate backbones of DNA?
A. helicase
B. ligase*
C. restriction enzyme
D. reverse transcriptase
Difficulty: Easy
ASM Standard: 26, 36
7. Genes to be cloned into a plasmid are typically inserted in which location?
A. in a location disrupting the antibiotic selective marker
B. oriC (origin of replication)
C. polylinker site*
D. randomly
Difficulty: Easy
ASM Standard: 19, 26, 36
Page 3 of 20
