LAB 5 Meat products BIOL 3150 Analyzing and isolating putative coliforms in meat samples using Eosin-Methylene Blue agar

Analyzing and isolating putative coliforms in meat samples using Eosin-Methylene Blue agar Tamkeen Habibi ID #: Saptarshi Sengupta BIOL 3150 LAB 05 Due: March 6th, 2019 1 This study source was downloaded by from CourseH on :32:29 GMT -05:00 Materials and Method The materials and methods section were followed exactly as stated in the laboratory manual of winter 2019 for all the solutions, and procedures to carry out experiments of exercise 3 in Lab 4 and Exercise 3 of Lab 5. Lab manual citation Noel, T.C., Coukell, M.B. and Nivillac N. 2016. SC/BIOL 3150 4.0 Microbiology Lab manual. York University. Pp 51-54 and 61-62 Results Table 1. Gram staining and streak plate method of the meat sample. The weight of the meat sample was aseptically weight out to be 1.01g in which it was mixed in saline solution and dilutions were taken. Gram staining and streak plate technique of the meat sample was performed using the 10^-2 dilutions. Using the microscope, it was shown to be rodded shape, pink in color and appeared in short chains or small clumps bumped together. Table 2. Plating cells for total counts and coliform counts. The weight of the meat sample was measured to be 1.01g in which it was vigorously mixed with saline solution by which the dilutions were taken. The nutrient agar deep was poured into the plates along with the prepared dilutions in which the colonies were counted after 2 Meat Samples Observations Drawi