LQB182 ONLINE LABORATORY WORKSHEET QUESTIONS WITH
COMPLETE SOLUTIONS
LQB182 Online Laboratory Worksheet
Name:
Student number:
Online Laboratory Task:
Practical V Racing
Chloroplasts
1. Complete your name and student number at the top.
2. Answer the questions below. 6 marks worth 6%.
Q1. RESULTS – these are the data you have obtained from the microscopy images. (1 mark)
You must show your working to gain full marks.
Total number of chloroplasts per cell from 6 independent counts (In equation 1, this value is
substituted for M)
Cell #1 Cell #2 Cell #3 Cell #4 Cell #5 Cell #6
Total Total Total Total Total Total
Chloroplasts Chloroplasts Chloroplasts Chloroplasts Chloroplasts Chloroplasts
19 20 18 32 27 34
Average number of chloroplasts (M) = (19+20+18+32+27+34)/5 = 25 chloroplast/cell
Determine the average diameter of the chloroplasts in micrometres (m) from 6 different
chloroplasts. Calculate the average radius in metres (In equation 1, this calculated value is
substituted for r).
Chloroplast 1 2 3 4 5 6
Diameter
4 5.5 6 5.5 4.5 5
(µm)
Average (mean) diameter in µm (4+5.5+6+5.5+4.5+5)/6 = ~5.08 = 5 µm
Average (mean) radius (r) in µm 5/2 = 2.5 µm
Convert r to SI units in m (metres)
2.5 x 10-6 m
Practical
This study source 5 RacingbyChloroplasts
was downloaded 100000844708667 from CourseHero.com on 11-03-2022 10:43:14 GMT -05:00 1
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, LQB182 Online Laboratory Worksheet
The average velocity of the chloroplasts in micrometres per second (m per second) was recorded
from 6 independent measurements (6 different chloroplasts) (In equation 1, this calculated value is
substituted for V)
Chloroplast 1 2 3 4 5 6
Velocity (µm/s)
4 5 5 6 8 2
Average velocity (V) in µm/s ((4+5+5+6+8+2) / 6) = 5 µm/s
Convert r to SI units in m/s (x106) = 5.0 x 10-6 m/s
Q2. Calculations (4 marks total)
Energy expended to drive cyclosis in a single chloroplast cell
Use the formula (Equation 1) derived in the practical module (included below) to calculate the
energy expended to bring about cyclosis in each of the cells of the leaves you have been observing.
Don’t forget units! Use brackets around each variable when performing your calculations.
You must show your working to gain full marks.
Equation 1: 6πrnV2M (Js-1 Cell-1 for a cell containing M chloroplasts)
π 3.14 (pi)
r 2.5 x 10-6 m (your mean radius)
n 10-2 Ns m-2 (viscosity for cytoplasm)
V 5.0 x10-6 m/s
M 25 (your mean chloroplast number)
Working:
= 6 x 3.14 x (2.5 x 10-6) x (10-2) x (5.0 x 10-6)2 x 25
= 18.84 x (2.5 x 10-8) x (2.5 x 10-11) x 25
= 2.944 x 10-16
Eq 1 Answer = 2.944 x 10-16 Joules per second per cell (Js-1 Cell-1)
Practical
This study source 5 RacingbyChloroplasts
was downloaded 100000844708667 from CourseHero.com on 11-03-2022 10:43:14 GMT -05:00 2
https://www.coursehero.com/file/68559990/LQB182-Prac-5docx/
COMPLETE SOLUTIONS
LQB182 Online Laboratory Worksheet
Name:
Student number:
Online Laboratory Task:
Practical V Racing
Chloroplasts
1. Complete your name and student number at the top.
2. Answer the questions below. 6 marks worth 6%.
Q1. RESULTS – these are the data you have obtained from the microscopy images. (1 mark)
You must show your working to gain full marks.
Total number of chloroplasts per cell from 6 independent counts (In equation 1, this value is
substituted for M)
Cell #1 Cell #2 Cell #3 Cell #4 Cell #5 Cell #6
Total Total Total Total Total Total
Chloroplasts Chloroplasts Chloroplasts Chloroplasts Chloroplasts Chloroplasts
19 20 18 32 27 34
Average number of chloroplasts (M) = (19+20+18+32+27+34)/5 = 25 chloroplast/cell
Determine the average diameter of the chloroplasts in micrometres (m) from 6 different
chloroplasts. Calculate the average radius in metres (In equation 1, this calculated value is
substituted for r).
Chloroplast 1 2 3 4 5 6
Diameter
4 5.5 6 5.5 4.5 5
(µm)
Average (mean) diameter in µm (4+5.5+6+5.5+4.5+5)/6 = ~5.08 = 5 µm
Average (mean) radius (r) in µm 5/2 = 2.5 µm
Convert r to SI units in m (metres)
2.5 x 10-6 m
Practical
This study source 5 RacingbyChloroplasts
was downloaded 100000844708667 from CourseHero.com on 11-03-2022 10:43:14 GMT -05:00 1
https://www.coursehero.com/file/68559990/LQB182-Prac-5docx/
, LQB182 Online Laboratory Worksheet
The average velocity of the chloroplasts in micrometres per second (m per second) was recorded
from 6 independent measurements (6 different chloroplasts) (In equation 1, this calculated value is
substituted for V)
Chloroplast 1 2 3 4 5 6
Velocity (µm/s)
4 5 5 6 8 2
Average velocity (V) in µm/s ((4+5+5+6+8+2) / 6) = 5 µm/s
Convert r to SI units in m/s (x106) = 5.0 x 10-6 m/s
Q2. Calculations (4 marks total)
Energy expended to drive cyclosis in a single chloroplast cell
Use the formula (Equation 1) derived in the practical module (included below) to calculate the
energy expended to bring about cyclosis in each of the cells of the leaves you have been observing.
Don’t forget units! Use brackets around each variable when performing your calculations.
You must show your working to gain full marks.
Equation 1: 6πrnV2M (Js-1 Cell-1 for a cell containing M chloroplasts)
π 3.14 (pi)
r 2.5 x 10-6 m (your mean radius)
n 10-2 Ns m-2 (viscosity for cytoplasm)
V 5.0 x10-6 m/s
M 25 (your mean chloroplast number)
Working:
= 6 x 3.14 x (2.5 x 10-6) x (10-2) x (5.0 x 10-6)2 x 25
= 18.84 x (2.5 x 10-8) x (2.5 x 10-11) x 25
= 2.944 x 10-16
Eq 1 Answer = 2.944 x 10-16 Joules per second per cell (Js-1 Cell-1)
Practical
This study source 5 RacingbyChloroplasts
was downloaded 100000844708667 from CourseHero.com on 11-03-2022 10:43:14 GMT -05:00 2
https://www.coursehero.com/file/68559990/LQB182-Prac-5docx/
