BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
Lab Notebook Bookmarks (click to
navigate): Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
Lab 1 Notebook
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Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with how to conduct
experiments and practice proper safety procedures while in a Lab. Cultivation, Identification,
and Evaluation are the 3 main principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of microbiology lab with an
introduction to basic equipment and safety rules. We are also show how to keep our Lab
Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer machine with a
thick door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
▪ Put tools in before you use for a lab OR put contaminated tools to
kill bacteria
▪ Not just dry air, steam is more efficient
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes and rotates culture in
a swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve samples and keep
them for long term.
• Test tubes which may have liquid broth, petri dishes which are
wrapped airtight, or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
➢ Latex (or nitrile (no latex) or thermal gloves (handle anything hot) or cold
gloves (liquid nitrogen)
➢ Eyewear protection
➢ Lab coat (worn when working with chemicals that can be spilled)
❖ Never leave lab while wearing PPE
➢ Spreads and containments other areas
▪ Public hallway, restrooms, cafeteria, etc.
Results: In this Lab, the instructor was able to educate us on proper Lab room procedures and
safety. We were given an introduction in learning the basic equipment we will use to cultivate,
identify, and examine bacteria.
Lab 2 Notebook
Back to Home Page
Title: Introduction to Microscopy
Objective:
To become familiar with the basic components of a light microscope & how to load a sample for
viewing.
Procedure:
Using standard light microscope.
1) Review parts & components
2) Load sample of glass slide onto microscope
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
(Microbiology) Lab Notebook latest
update 2022/2023
Lab Notebook Bookmarks (click to
navigate): Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
Lab 1 Notebook
Back to Home Page
Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with how to conduct
experiments and practice proper safety procedures while in a Lab. Cultivation, Identification,
and Evaluation are the 3 main principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of microbiology lab with an
introduction to basic equipment and safety rules. We are also show how to keep our Lab
Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer machine with a
thick door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
▪ Put tools in before you use for a lab OR put contaminated tools to
kill bacteria
▪ Not just dry air, steam is more efficient
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes and rotates culture in
a swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve samples and keep
them for long term.
• Test tubes which may have liquid broth, petri dishes which are
wrapped airtight, or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
, BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
➢ Latex (or nitrile (no latex) or thermal gloves (handle anything hot) or cold
gloves (liquid nitrogen)
➢ Eyewear protection
➢ Lab coat (worn when working with chemicals that can be spilled)
❖ Never leave lab while wearing PPE
➢ Spreads and containments other areas
▪ Public hallway, restrooms, cafeteria, etc.
Results: In this Lab, the instructor was able to educate us on proper Lab room procedures and
safety. We were given an introduction in learning the basic equipment we will use to cultivate,
identify, and examine bacteria.
Lab 2 Notebook
Back to Home Page
Title: Introduction to Microscopy
Objective:
To become familiar with the basic components of a light microscope & how to load a sample for
viewing.
Procedure:
Using standard light microscope.
1) Review parts & components
2) Load sample of glass slide onto microscope
BIO 171-Portage Learning
(Microbiology) Lab Notebook latest
update 2022/2023
